Evaluation of Anti-ulcer Activity of Hydro Alcoholic Extracts of Abutilon indicum, Helianthus annuus and Combination of Both against Ethanol and Pyloric Ligation Induced Gastric Ulcer in Albino Wistar Rats
Kambham Venkateswarlu *
Department of Pharmaceutics, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India.
K. Vijayabhaskar
Department of Pharmacology, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India.
O. Sreenivasa Krishna
Department of Pharmacology, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India.
N. Devanna
Department of Chemistry, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India.
K. B. Chandra Sekhar
Department of Chemistry, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu-515 002, Andhra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Abutilon indicum and Helianthus annuus consists of various compounds which have different therapeutic activities.
Objective: The main aim of the present study was to compare the anti-ulcer activity of hydroalcoholic extracts of A. indicum, H. annuus and combination of both against Ethanol induced gastric ulcer and pyloric ligation induced gastric ulcer in Albino Wistar Rats.
Methodology: The method used was OECD 423 (Acute Toxic Class Method), which allowed evaluating the toxic dose of the crude drugs. Furthermore, the method adopted, provided the selection of proper dose regime to investigate the activity against both types of stomach ulcers in animal models. The omeprazole was used as standard drug for comparison.
Results: Three different extracts were prepared for evaluation namely: HAEAI, HAEHA and C-HAEAIHA which showed a statistically significant anti-ulcer activity. The protective effect was found to be in the following order: C-HAEAIHA > HAEHA > HAEAI.
Conclusion: Among the tested extracts, the combined mixture of hydroalcoholic extract of A. indicum and H. annuus (C-HAEAIHA) showed better anti-ulcer activity.
Keywords: Acute Toxic Class Method, Abutilon indicum, Helianthus annuus, stomach anti-ulcer activity, animal model.