A Validated Bioanalytical Method for Quantification of Ziprasidone in Rabbit Plasma by LC-MS/MS: Application to a Pharmacokinetic Study
Khagga Bhavyasri *
Department of Center for Pharmaceutical Sciences, J.N.T. University, Kukatpally, Hyderabad, 500 072, A.P, India.
V. Murali Balaram
Department of Center for Pharmaceutical Sciences, J.N.T. University, Sultan Ul-Uloom College of Pharmacy, Hyderabad, A.P, India.
R. Nageswarao
Department of Chemistry Indian Institute of Chemical Sciences, Tarnaka, Hyderabad-500 072, A.P, India.
D. Rambabu
Agilent Technologies India Pvt. Ltd., Hyderabad, A.P, India.
M. Ajitha
Department of Center for Pharmaceutical Sciences, J.N.T. University, Kukatpally, Hyderabad, 500 072, A.P, India.
Bala Sekhara Reddy Challa
Department of Pharmaceutical Analysis, Acharya Nagarjuna University, Vagdevi College of Pharmacy, Gurazala, Andhrapradesh, 522415, India.
*Author to whom correspondence should be addressed.
Abstract
A Liquid-liquid extraction method was developed for quantification of Ziprasidone in Rabbit plasma by a suitable bio-analytical method with LC-MS/MS. Ziprasidone d8 was used as an internal standard for Ziprasidone. Hypurity C18, 150 x 4.6 mm, 5 µm column used for chromatographic separation of analyte followed by detection with mass spectrometry. The total run time was 4.0 minutes. The proposed method has been validated with the linear range of 0.05 – 200.00 ng/mL for Ziprasidone. The intra-run and inter-run precision values were within 0.625 to 0.947% and 2.182 to 3.198% for Ziprasidone. The overall recovery for Ziprasidone and Ziprasidone d8 was 92.57% and 95.70% respectively. This validated method was successfully applied into the pharmacokinetic study of Rabbit plasma.
Keywords: Ziprasidone, Rabbit plasma, mass spectrometry, pharmacokinetic