Determination of Phenolic Contents by HPLC, and Antioxidant, Antimicrobial, Antityrosinase, and Anticholinesterase Activities of Psephellus huber-morathii
Nuriye Korkmaz
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Sıla Ozlem Sener
Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Nuray Balturk
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Seyda Kanbolat
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Merve Badem *
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Rezzan Aliyazicioglu
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Ufuk Ozgen
Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
Ali Kandemir
Department of Biology, Faculty of Science, Erzincan University, 24000 Erzincan, Turkey.
Sengul Alpay Karaoglu
Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
*Author to whom correspondence should be addressed.
Abstract
The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. The antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. The methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity.
Keywords: Antioxidant, antimicrobia, anticholinesterase, Psephellus huber-morathii