Modulation the Neuro-toxicity Induced by Aluminum Chloride in Rats Using Beetroots and Broccli Extracts
Abeer Ali Al-Balawi
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Yousri Mohamed Ahmed
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, Production of Bioproducts for Industrial Applications Research Group and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Saudi Arabia and Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Division, National Research Center, Dokki–Cairo, Egypt.
Ashwag Albukhari
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Shareefa A. ALGhamdi
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Mustafa A. Zeyadi
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Morog R. Maddah
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Etimad H. Huwait
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Soad Ali
Department of Anatomy, Yousef Abdullatif Jameel Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Taha A. Kumosani
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia and Production of Bioproducts for Industrial Applications Research Group and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Saudi Arabia.
Said S. Moselhy *
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, Bioactive Natural Products Research Group, King Abdulaziz University, Saudi Arabia and Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Backgound: The generation of oxidative stress can be referred to Aluminium toxic effect in animals and humans. This study aimed to evaluate the role of broccoli (Br) and beetroot (Be) extarcts as antioxidant that prevents oxidative stress that associated with aluminum toxicity.
Materials and Methods: Fifty Wister female rats were grouped into five groups (each 10 rats): Group 1: control group, administered drinking water only. Group 2: (Neurogenerative) which were induced by oral administration of aluminum chloride (20 mg/kg b.w) daily for one month. Group 3: Rats given aluminum chloride were treated with Rivastigmine (Ri) (1 mg/kg b.w) as a reference drug daily for five weeks. Group 4: Rats given aluminum chloride were treated with beet root extract (50 mg/kg b.w) daily for six weeks. Group 5. Rats given aluminum chloride were treated with broccoli extract (50 mg/kg b.w) daily for five weeks.
Results: (AlCl3) group showed a significant increase in Ach level (P<0.05) and a non-significant change in DOP and NE levels compared to control. (AlCl3+Be) was non-significant (P˂0.05) change in Ach, DOP and NE levels compared to (AlCl3) group and showed a significant (P<0.05) increase in Ach level compared to control. (AlCl3+Br) showed a significant (P<0.05) increase in NE level and non-significant (P˂0.05) change in Ach and DOP levels compared to (AlCl3) group. (AlCl3+Ri) showed a significant (P<0.05) increase in Ach, DOP and NE levels compared to (AlCl3) group. Also, showed a significant (P<0.05) increase in Ach and NE compared to control.
Conclusion: Neuroprotective role of broccoli in the present study which may result from its antioxidant properties due to its bioactive content such as glucosinolate, isothiocyanate, Sulforaphane, and flavonoids. Therefore, Broccoli can have a favorable effect on neurotoxicity due to their antioxidant and anti-inflammatory properties.
Keywords: Neurotoxicity, Brocolli, beetroots, antioxidants.