The antibacterial activity, in-silico DNA molecular docking, DNA binding and photocleavage studies of newly synthesized pyrazole is described. Antibacterial potential of these compounds screened against a wide range of Gram-positive and Gram-negative bacteria showed significant zone of inhibition and MIC with standard drug ciproflaxin is investigated. Among all the orientation of binding, fourth orientation showed significant binding and revealed that the binding and docking energy of 4a was -8.62 and -8.66 and inhibition constant 7.46 X e-6. The absorption spectra showed the dynamic interaction with CT DNA and as proficient DNA intercalator (Kb = 4.5×104 M-1). The viscosity measurements and thermal denaturation affords the positive results towards DNA intercalation in both the studies.. The light induced DNA damage was pragmatic in the absence of various ‘‘inhibitors’’ shows in photo cleavage activities at 360 nm.
Tabernaemontana divaricata (L.) R.Br belonging to Apocynaceae family is traditionally used by people in many parts of the world to treat various disorders. The present study was undertaken to investigate anti-ulcer property of Tabernaemontana divaricata flower methanolic extract (TDFME 500 mg/kg, p.o) by pyloric ligation induced gastric ulceration model using Omeprazole (8mg/kg, p.o) as a standard drug in wistar rats. Five parameters i.e., volume of gastric juice, pH, free & total acidities and ulcer index were assessed. The test extract significantly (p< 0.01) decreased volume of gastric juice, free & total acidities and ulcer index. Like standard, it also raised pH of gastric acid. The observed percentage protection for standard and test were 89.84% and 79.53%, respectively. Thus, TDFME 500 mg/kg had a positive effect on all the parameters under study and the results were similar to that of standard. From the above results, it can be concluded that TDFME exhibits remarkable gastroprotective effect.
All clinicians should be fully aware of the recent trends in their speciality to enable them to provide effective and successful treatment to their patients. One vital aspect of the treatment is that the clinician should constantly update his knowledge on the drugs being administered during the course of treatment and their interactions. The purpose of this article is to review the current pharmacological agents being used in Prosthodontics along with their interactions and indications. The paper mainly focuses on Therapeutic drugs and drugs that aid in prosthodontics treatment. Therapeutic drugs include local anesthetics, antiseptics, steroids, analgesics, antimicrobials, antifungals, antianxity drugs, centrally acting muscel relaxants. Drugs that aid in prosthodontics treatment include astringents, vasoconstrictors, hemostatic agents, sialogogues, anti-sialogogues, denture cleansers, gum paints, denture cleansers, denture adhesives, ORAL protective agents and demulcents. An odontologist should have sound knowledge of the benefits and drawbacks of all these agents. This will enable the clinician to provide a safe and predictable treatment to the patients.