In vitro Antiglycation, Antioxidant and Antiproliferative Properties of Peptides Derived from Tryptic Hydrolysis of Soya Bean
Abudukadeer Kuerban *
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Said S. Moselhy
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia and Bioactive Natural Products Research Group, KAU, Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Jeddah, Kingdom of Saudi Arabia and Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Hanaa Ghashlan
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Taha A. Kumosani
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Jeddah, Kingdom of Saudi Arabia and Production of Natural Products for Industrial Health Research Group, KAU, Jeddah, Kingdom of Saudi Arabia.
Othman A. S. Baothman
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Mustafa Zeyadi
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Nawal Helmi
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Tahani M. Alghamdi
Collage of Pharmacy, Ibin Sina Medical Collage, Jeddah, Kingdom of Saudi Arabia.
Mazin A. Zamzami
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia.
Muqtadir Baig Mirza
Department of Biological Science, Faculty of Science, KAU, Jeddah, Kingdom of Saudi Arabia.
Fareeduddin Quadri Syed
Department of Biological Science, Faculty of Science, KAU, Jeddah, Kingdom of Saudi Arabia.
Furkhan Ahmed Mohammed
Department of Biological Science, Faculty of Science, KAU, Jeddah, Kingdom of Saudi Arabia.
Abdulrahman L. AL-Malki
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia and Bioactive Natural Products Research Group, KAU, Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Jeddah, Kingdom of Saudi Arabia.
Khalid O. Abulnaja
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia and Bioactive Natural Products Research Group, KAU, Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Jeddah, Kingdom of Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
Aims: Bioactive peptides are acknowledged for their vital contributions to health promotion. This study aims to evaluate antiglycation, antioxidant and antiproliferative ability of peptides derived from tryptic hydrolysis of Soya bean.
Place and Duration of Study: Department of Biochemistry, Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, between September 2016 and June 2017.
Methodology: Hydrolysis profile was qualified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Reducing sugars-trapping ability of peptides was examined. 0.5% methacrylamido phenylboronic acid (MPBA) gel was used to investigate the advanced glycation end products (AGEs) inhibition. The in vitro antioxidant activity of peptides were tested by 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay. MCF-7, PC3, and HepG2 cell lines were used to explore the antiproliferative potency of peptides.
Results: Peptide fractions trapped reducing sugars and reduced AGEs formation, and exhibited well DPPH scavenging ability with IC50 8.96 mg/mL. Peptide fractions showed dose-dependent toxicity to the used cell lines.
Conclusion: Released bioactive peptides emphasized the value of Soya bean proteins to be used as a functional food or alternative supplementary to diseases.
Keywords: Bioactive peptides, methacrylamido phenylboronic acid, antioxidant, antiglycation, antiproliferative, glycation