Journal of Pharmaceutical Research International

Aim: To evaluate the antidiabetic potential of Putranjiva roxburghii leaves using In vitro models, focusing on their ability to inhibit key enzymes involved in carbohydrate metabolism.

Study Design: The current research program is an attempt to screen In vitro anti-diabetic activity of ethanolic extract of Putranjiva roxburghii wall leaves by using – α amylase inhibitory activity and starch iodine assay.

Place and Duration of Study: Department of Pharmacology, SJM College of Pharmacy Chitradurga, between July to October.  2024

Methodology: In this study, the crude extract was obtained by using the soxhlet extraction method using 70% ethanol, The extract was used to analyse the antidiabetic activity using in-vitro models namely α-amylase inhibition activity and starch iodine assay, all the experiments were performed in triplicates. The effect of leaf extract was compared with the standard drug metformin.

Results: The results of α-amylase inhibition activity showed that the extract inhibits the α amylase enzyme in a concentration (0.3,0.6,0.9,1.2 and 1.5mg/ml) with a percentage inhibition range (6.02%-55.55%) for extract which is not significantly different as compared to metformin (16.12% - 69.89%). Similarly, for starch iodine assay the extract inhibits the α-amylase enzyme in a concentration (same as above) with a percentage inhibition range (16.66%-75%) for extract which is not significantly different as compared to metformin (22.57% - 85.71%).

Conclusion: From the above finding of the study, Putranjiva roxburghii wall leaf extract has the ability to inhibit α-amylase enzyme and exhibits antidiabetic activity.