Bio-fabrication and Characterization of Green Synthesized Nanoparticles from Commercial Honey

Najla Ali Alburae *

Department of Biological Sciences, King Abdulaziz University, P.O. Box 80206, 21589, Jeddah, Saudi Arabia.

*Author to whom correspondence should be addressed.


Abstract

Green approaches to nanoparticle synthesis offer sustainable and environmentally friendly alternatives, avoiding hazardous chemicals typical in traditional methods. This study characterizes nanoparticles (NPs) synthesized from silver nitrate (AgNO3) and iron oxide (Fe2O3) using commercial honey as a reducing and capping agent. Characterization revealed significant disparities between silver NPs (AgNPs) and iron NPs (FeNPs). AgNPs had a larger particle size (Z-average: 3115.67 nm) compared to FeNPs (Z-average: 1813 nm). AgNPs showed a monodisperse population, while FeNPs had a slightly broader size distribution. Additionally, AgNPs had a higher particle concentration (mean count rate: 505.17 kcps) than FeNPs (mean count rate: 296.65 kcps). Both AgNPs and FeNPs displayed negative surface charges, at -6.499 mV and -1.652 mV, respectively, where FeNPs exhibit a slightly higher value. Elemental composition analysis by scanning electron microscope – energy dispersive X-ray (SEM-EDX) revealed that AgNPs are primarily composed of silver, carbon, and oxygen, whereas FeNPs consisted mainly of iron, oxygen, and carbon. These findings provide insights into the physical and chemical properties of AgNPs and FeNPs synthesized using commercial honey. Understanding these properties is essential for optimizing synthesis processes and exploring applications in medicine, catalysis, and environmental remediation. The eco-friendly synthesis approach using honey underscores the potential for sustainable nanomaterial production. Further research can explore specific applications and benefits of AgNPs and FeNPs synthesized through this green method, offering an efficient and economical alternative for nanoparticle synthesis.

Keywords: Nanoparticle synthesis, green approach, commercial honey, characterization, AgNPs, FeNPs


How to Cite

Alburae, N. A. (2024) “Bio-fabrication and Characterization of Green Synthesized Nanoparticles from Commercial Honey”, Journal of Pharmaceutical Research International, 36(6), pp. 77–87. doi: 10.9734/jpri/2024/v36i67524.

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References

Rostamizadeh E, Iranbakhsh A, Majd A, Arbabian S, Mehregan I. Green synthesis of Fe2O3 nanoparticles using fruit extract of Cornus mas L. and its growth-promoting roles in Barley. J Nanostruct Chem. 2020;10(2):125-130. DOI:10.1007/s40097-020-00335-z

Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2014;9(6):385-406.

Galúcio JMP, De Souza SGB, Vasconcelos AA, et al. Synthesis, Characterization, Applications, and Toxicity of Green Synthesized Nanoparticles. CPB. 2022;23(3):420-443. DOI:10.2174/1389201022666210521102307

Mohammadzadeh V, Barani M, Amiri MS, et al. Applications of plant-based nanoparticles in nanomedicine: A review. Sustainable Chemistry and Pharmacy. 2022;25:100606. DOI: 10.1016/j.scp.2022.100606

Nagajyothi PC, Pandurangan M, Kim DH, Sreekanth TVM, Shim J. Green Synthesis of Iron Oxide Nanoparticles and Their Catalytic and In vitro Anticancer Activities. J Clust Sci. 2017;28(1):245- 257. DOI: 10.1007/s10876-016-1082-z

Jamkhande PG, Ghule NW, Bamer AH, Kalaskar MG. Metal nanoparticles synthesis: An overview on methods of preparation, advantages and disadvantages, and applications. Journal of Drug Delivery Science and Technology. 2019;53:101174. DOI: 10.1016/j.jddst.2019.101174

Rathod S, Preetam S, Pandey C, Bera SP. Exploring synthesis and applications of green nanoparticles and the role of nanotechnology in wastewater treatment. Biotechnology Reports. 2024; 41:e00830. DOI: 10.1016/j.btre.2024.e00830

Chugh D, Viswamalya VS, Das B. Green synthesis of silver nanoparticles with algae and the importance of capping agents in the process. Journal of Genetic Engineering and Biotechnology. 2021;19 (1):126. DOI: 10.1186/s43141-021-00228-w

Shah AA, Bhatti MA, Tahira A, et al. Facile synthesis of copper doped ZnO nanorods for the efficient photo degradation of methylene blue and methyl orange. Ceramics International. 2020;46(8):9997-10005. DOI: 10.1016/j.ceramint.2019.12.024

Gopu M, Kumar P, Selvankumar T, et al. Green biomimetic silver nanoparticles utilizing the red algae Amphiroa rigida and its potent antibacterial, cytotoxicity and larvicidal efficiency. Bioprocess Biosyst Eng. 2021;44(2):217-223. DOI: 10.1007/s00449-020-02426-1

Üstün E, Önbaş SC, Çelik SK, Ayvaz MÇ, Şahin N. Green Synthesis of Iron Oxide Nanoparticles by Using Ficus Carica Leaf Extract and Its Antioxidant Activity. Biointerface Res Appl Chem. 2021;12(2): 2108-2116. doi:10.33263/BRIAC122.21082116

González Fá AJ, Juan A, Di Nezio MS. Synthesis and Characterization of Silver Nanoparticles Prepared with Honey: The Role of Carbohydrates. Analytical Letters. 2017;50(5):877-888. DOI: 10.1080/00032719.2016.1199558

Behravan M, Hossein Panahi A, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules. 2019;124: 148-154. DOI: 10.1016/j.ijbiomac.2018.11.101

Rasheed T, Bilal M, Iqbal HMN, Li C. Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids and Surfaces B: Biointerfaces. 2017;158:408-415. DOI: 10.1016/j.colsurfb.2017.07.020

Aboyewa JA, Sibuyi NRS, Meyer M, Oguntibeju OO. Green Synthesis of Metallic Nanoparticles Using Some Selected Medicinal Plants from Southern Africa and Their Biological Applications. Plants. 2021;10(9):1929. DOI: 10.3390/plants10091929

Varadavenkatesan T, Selvaraj R, Vinayagam R. Phyto-synthesis of silver nanoparticles from Mussaenda erythrophylla leaf extract and their application in catalytic degradation of methyl orange dye. Journal of Molecular Liquids. 2016;221:1063-1070. DOI: 10.1016/j.molliq.2016.06.064

Tufani A, Qureshi A, Niazi JH. Iron oxide nanoparticles based magnetic luminescent quantum dots (MQDs) synthesis and biomedical/biological applications: A review. Materials Science and Engineering: C. 2021;118:111545. DOI: 10.1016/j.msec.2020.111545

Ali I, Pan Y, Jamil Y, et al. Comparison of copper-based Cu-Ni and Cu-Fe nanoparticles synthesized via laser ablation for magnetic hyperthermia and antibacterial applications. Physica B: Condensed Matter. 2023;650:414503. DOI: 10.1016/j.physb.2022.414503

Keskin M, Kaya G, Keskin S. Nanotechnology in Honey: Future and Perspectives Honey as Nanoparticles. In: Bhattacharya T, Ahmed S, eds. Nanotechnology in Functional Foods. 1st ed. Wiley. 2022:87-101. DOI: 10.1002/9781119905059.ch4

Haiza H, Azizan A, Mohidin AH, Halin DSC. Green Synthesis of Silver Nanoparticles Using Local Honey. NH. 2013;4:87-98. doi:10.4028/www.scientific.net/NH.4.87

Shahid H, Shah AA, Shah Bukhari SNU, et al. Synthesis, Characterization, and Biological Properties of Iron Oxide Nanoparticles Synthesized from Apis mellifera Honey. Molecules. 2023;28(18): 6504. DOI: 10.3390/molecules28186504

Bhuiyan MdSH, Miah MY, Paul SC, et al. Green synthesis of iron oxide nanoparticle using Carica papaya leaf extract: application for photocatalytic degradation of remazol yellow RR dye and antibacterial activity. Heliyon. 2020;6(8):e04603. DOI: 10.1016/j.heliyon.2020.e04603

Abd El-Aziz ARM, Al-Othman MR. Biosynthesized gold nanoparticles using Zingiber officinale and its impact on the growth and chemical composition of lentils (Lens culinaris). PAKJBOT. 2019;51(2). DOI: 10.30848/PJB2019-2(21)

Scimeca M, Bischetti S, Lamsira HK, Bonfiglio R, Bonanno E. Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. Eur J Histochem. Published; 2018. DOI: 10.4081/ejh.2018.2841

Priya, Naveen, Kaur K, Sidhu AK. Green Synthesis: An Eco-friendly Route for the Synthesis of Iron Oxide Nanoparticles. Front Nanotechnol. 2021;3:655062. DOI: 10.3389/fnano.2021.655062

El-Desouky T, Ammar H. Honey mediated silver nanoparticles and their inhibitory effect on aflatoxins and ochratoxin A. J App Pharm Sci. Published online 2016: 083-090. DOI: 10.7324/JAPS.2016.60615

Wan Y, Guo Z, Jiang X, et al. Quasi-spherical silver nanoparticles: Aqueous synthesis and size control by the seed-mediated Lee–Meisel method. Journal of Colloid and Interface Science. 2013;394: 263-268. DOI: 10.1016/j.jcis.2012.12.037

Kumar R, Goswami S, Rai GK, et al. Protection from terminal heat stress: a trade-off between heat-responsive transcription factors (HSFs) and stress-associated genes (SAGs) under changing environment. Cereal Research Communications. 2020;49(2):227-234. DOI: 10/gsm75g

Mohammed A, Al-Qahtani A, al-Mutairi A, Al-Shamri B, Aabed K. Antibacterial and Cytotoxic Potential of Biosynthesized Silver Nanoparticles by Some Plant Extracts. Nanomaterials. 2018;8(6):382. DOI: 10.3390/nano8060382

Farhadi S, Ajerloo B, Mohammadi A. Green Biosynthesis of Spherical Silver Nanoparticles by Using Date Palm (Phoenix Dactylifera) Fruit Extract and Study of Their Antibacterial and Catalytic Activities. Acta Chim Slov; 129-143. DOI: 10.17344/acsi.2016.2956

Khatoon N, Mazumder JA, Sardar M. Biotechnological Applications of Green Synthesized Silver Nanoparticles. J Nanosci Curr Res. 2017;02(01). DOI: 10.4172/2572-0813.1000107

Balasooriya ER, Jayasinghe CD, Jayawardena UA, Ruwanthika RWD, Mendis De Silva R, Udagama PV. Honey Mediated Green Synthesis of Nanoparticles: New Era of Safe Nanotechnology. Journal of Nanomaterials. 2017;2017:1-10. DOI: 10.1155/2017/5919836

Gengan RM, Anand K, Phulukdaree A, Chuturgoon A. A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf. Colloids and Surfaces B: Biointerfaces. 2013;105: 87-91. DOI: 10.1016/j.colsurfb.2012.12.044

Guo Y, Zhao Y, Wang S, Jiang C, Zhang J. Relationship between the zeta potential and the chemical agglomeration efficiency of fine particles in flue gas during coal combustion. Fuel. 2018;215:756 -765. DOI: 10.1016/j.fuel.2017.11.005

El-Deeb N, El-Sherbiny I, El-Aassar M, Hafez E. Novel Trend in Colon Cancer Therapy Using Silver Nanoparticles Synthesized by Honey Bee. J Nanomed Nanotechnol. 2015;06(02). DOI: 10.4172/2157-7439.1000265

Femi-Adepoju AG, Dada AO, Otun KO, Adepoju AO, Fatoba OP. Green synthesis of silver nanoparticles using terrestrial fern (Gleichenia Pectinata (Willd.) C. Presl.): characterization and antimicrobial studies. Heliyon. 2019;5(4): e01543. DOI: 10.1016/j.heliyon.2019.e01543

Matar GH, Akyüz G, Kaymazlar E, Andac M. An Investigation of Green Synthesis of Silver Nanoparticles Using Turkish Honey Against Pathogenic Bacterial Strains. Biointerface Res Appl Chem. 2022;13(2): 195. DOI: 10.33263/BRIAC132.195

Hosny AMS, Kashef MT, Rasmy SA, Aboul-Magd DS, El-Bazza ZE. Antimicrobial activity of silver nanoparticles synthesized using honey and gamma radiation against silver-resistant bacteria from wounds and burns. Adv Nat Sci: Nanosci Nanotechnol. 2017;8(4):045009. DOI: 10.1088/2043-6254/aa8b44

Miri A, Najafzadeh H, Darroudi M, Miri MJ, Kouhbanani MAJ, Sarani M. Iron Oxide Nanoparticles: Biosynthesis, Magnetic Behavior, Cytotoxic Effect. Chemistry Open. 2021;10(3):327-333. DOI: 10.1002/open.202000186

Salama AM, Abedin RMA, Elwakeel KZ. Influences of greenly synthesized iron oxide nanoparticles on the bioremediation of dairy effluent using selected microbial isolates. Int J Environ Sci Technol. 2022; 19(8):7019-7030. DOI: 10.1007/s13762-021-03625-3

Ahmad W, Kumar Jaiswal K, Amjad M. Euphorbia herita leaf extract as a reducing agent in a facile green synthesis of iron oxide nanoparticles and antimicrobial activity evaluation. Inorganic and Nano-Metal Chemistry. 2020:1-8. DOI: 10.1080/24701556.2020.1815062

Sharmila M, Mani RJ, Parvathiraja C, et al. Photocatalytic Dye Degradation and Bio-Insights of Honey-Produced α-Fe2O3 Nanoparticles. Water. 2022;14(15):2301. DOI: 10.3390/w14152301

Biswas A, Vanlalveni C, Lalfakzuala R, Nath S, Rokhum SL. Mikania mikrantha leaf extract mediated biogenic synthesis of magnetic iron oxide nanoparticles: Characterization and its antimicrobial activity study. Materials Today: Proceedings. 2021;42:1366-1373. DOI: 10.1016/j.matpr.2021.01.108

Menazea AA, Ahmed MK. Silver and copper oxide nanoparticles-decorated graphene oxide via pulsed laser ablation technique: Preparation, characterization, and photoactivated antibacterial activity. Nano-Structures & Nano-Objects. 2020;22: 100464. DOI: 10.1016/j.nanoso.2020.100464

Qasim S, Zafar A, Saif MS, et al. Green synthesis of iron oxide nanorods using Withania coagulans extract improved photocatalytic degradation and antimicrobial activity. Journal of Photochemistry and Photobiology B: Biology. 2020;204:111784. DOI: 10.1016/j.jphotobiol.2020.111784