Prevalence of Candida Species Isolated from Immuno-Compromised Patients

Sunpreet Kaur

Department of Microbiology, Index Medical College, Malwanchal University, Indore-451010, (M.P), India.

Madhurendra Singh Rajput *

Department of Microbiology, Amaltas Medical College, Malwanchal University, Dewas-455001, (M.P), India.

Balveer Singh Dhami

Department of Zoology, Bareilly College, Rohilkhand University, Bareilly-234001, (U.P), India.

*Author to whom correspondence should be addressed.


Background: Resistance to fungal infections is increasing worldwide, which is highly relevant in immunosuppressed individuals. Candida fungi infection constitutes one of the most common causes of fungal infections in such patients, and it can lead to complications and death. The current study sought to examine the prevalence and species diversity of Candida in samples received by the Microbiology Laboratory over a two-year period.

Methods: The study involved 674 immunocompromised patients. Candida was isolated from clinical samples using wet mount, gram stain, and SDA culture. To further identify the organisms, several tests were conducted, including the germ tube test, cornmeal agar morphology, sugar assimilation, fermentation tests, and BACT/ALERT 3D.

Results: Candida species were found to be present in 6.5% of the population. Candida tropicalis was found to be the most common isolate 20 (45.45%), followed by Candida albicans 9 (20.45%), Candida glabrata 7 (15.9%), Candida parapsilosis 4 (9.09%), and Candida krusei 4 (9.09%). Diabetes, leukaemia, chronic kidney disease, and AIDS were the most common predisposing factors for candidiasis.

Conclusion: Men had a relatively high candidemia prevalence of 68.18%. Uncontrolled diabetes mellitus (DM) and chronic kidney disease (CKD) have been found to be the most common co-morbidities with candidemia in people aged 51 to 70.

Keywords: Candida, immuno-compromised, fungal infection, candidemia, chromagar

How to Cite

Kaur, S., Rajput , M. S. and Dhami , B. S. (2023) “Prevalence of Candida Species Isolated from Immuno-Compromised Patients”, Journal of Pharmaceutical Research International, 35(14), pp. 1–9. doi: 10.9734/jpri/2023/v35i147369.


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Castanheira M, Deshpande LM, Davis AP, Rhomberg PR, and Pfaller MA. Monitoring antifungal resistance in a global collection of invasive yeasts and molds: Application of CLSI epidemiological cutoff values and whole-genome sequencing analysis for detection of azole resistance in Candida albicans. Antimicrobial Agents and Chemotherapy. 2017;61(10):e00906-17.

Pfaller MA, Rhomberg PR, Messer SA, Jones RN and Castanheira M. Isavuconazole, micafungin, and 8 comparator antifungal agents' susceptibility profiles for common and uncommon opportunistic fungi collected in 2013: temporal analysis of antifungal drug resistance using CLSI species-specific clinical breakpoints and proposed epidemiological cutoff values. Diagnostic Microbiology and Infectious Disease. 2015;82(4):303-13.

Alkharashi N, Aljohani S, Layqah L, Masuadi E, Baharoon W, Al-Jahdali H, Baharoon S. Candida Bloodstream Infection: Changing pattern of occurrence and antifungal susceptibility over 10 years in a tertiary care Saudi Hospital. Candida Journal of Infectious Diseases and Medical Microbiology. 2019;2015692.

Zarei Mahmoudabadi A, Rezaei-Matehkolaei A, Ghanavati F. The susceptibility patterns of Candida species isolated from urine samples to posaconazole and caspofungin. Jundishapur Journal of Microbiology. 2015;8(3):e24298.

Shekari Ebrahim Abad H, Zaini F, Kordbacheh P, Mahmoudi M, Safara M, and Mortezaee V. In Vitro Activity of Caspofungin Against Fluconazole-Resistant Candida Species Isolated From Clinical Samples in Iran. Jundishapur Journal of Microbiology. 2015;8(6):e18353.

Jensen RH, Johansen HK, Søes LM, Lemming LE, Rosenvinge FS, Nielsen L, Olesen B, Kristensen L, Dzajic E, Astvad KM and Arendrup MC. Posttreatment antifungal resistance among colonizing candida isolates in candidemia patients: Results from a systematic multicenter study. Antimicrobial Agents of Chemotherapy. 2015;60(3):1500-8.

Pappas P, Lionakis M, Arendrup M. et al. Invasive candidiasis. Nat Rev Dis Primers. 2018;4:18026.


Teoh F, Pavelka N. How chemotherapy increases the risk of systemic candidiasis in cancer patients: Current paradigm and future directions. Pathogens. 2016; 5(1):6.

Berkow EL, Lockhart SR, Ostrosky-Zeichner L. Antifungal susceptibility testing: Current approaches. Clinical Microbiology Reviews. 2020;33(3):e00069-19.

Thomas M, Oberoi A, Dewan E. Species distributionand antifungal susceptibility of candidemia at a multispecialty center in North India. CHRISMED Journal of Health and Research. 2016;3(1):33-36.

Gandham NR, Vyawahare CR, Jadhav SV, Misra RN. Candidemia: Speciation and antifungal susceptibility testing from a tertiary care hospital in Maharashtra, India. Medical Journal of Dr. DY Patil University. 2016;9(5):596-599.

Tak V, Mathur P, Varghese P, Gunjiyal J, Xess I and Misra MC. The epidemiological profile of candidemia at an Indian trauma care center. Journal of Laboratory Physicians. 2014;6(2):96-101.

Bongomin F, Gago S, Oladele RO and Denning DW. Global and multi-national prevalence of fungal diseases-estimate precision. Journal of Fungi (Basel). 2017;3(4):57.

Alkharashi N, Aljohani S, Layqah L, Masuadi E, Baharoon W, Al-Jahdali H, Baharoon S. Candida bloodstream infection: Changing pattern of occurrence and antifungal susceptibility over 10 years in a tertiary care Saudi Hospital. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019;2019:2015692.

Rodrigues CF, Rodrigues ME, Henriques M. Candida sp. infections in patients with diabetes mellitus. Journal of Clinical Medicine. 2019;8(1):76.

Phu TT, Truong NN, Phuong NT, Bup PH, Thao PT. Antifungal resistance of Candida Spp. in bloodstream infection. Biomedical Journal of Scientific and Technical Research. 2020;24(2):18153-18155.

Gade N, Neral A, Monga N, Aradhey P, Singh R, Barapatre R, Sherwani N, Joshi SG. Antifungal susceptibility pattern of clinical isolates of candida from a tertiary care Hospital in Chhattisgarh, India. Saudi Journal of Pathology and Microbiology. 2019;4(12):906-913.

Alagiri SB, Vijayaraman RS, Ramaraj V and Kindo AJ.Invasive yeast infections in the intensive care unit of a tertiary care centre in South India. Journal of the Academy of Clinical Microbiologists. 2017;19(1):19-26.

Rajeevan S, Thomas M, Appalaraju B. Characterization and antifungal susceptibility pattern of Candida species isolated from various clinical samples at a tertiary care Centre in South India. Indian Journal of Microbiology Research. 2016;3(1):53-57.

Tulasidas S, Rao P, Bhat S and Manipura R. A study on biofilm production and antifungal drug resistance among Candida species from vulvovaginal and bloodstream infections. Infection and Drug Resistance. 2018;11:2443-2448.

Husni R, Bou Zerdan M, Samaha N, Helou M, Mahfouz Y, Saniour R, Hourani S, Kolanjian H, Afif C, Azar E, El Jisr T, Mokhbat J, Abboud E, Feghali R, Abboud E, Matta H, Karayakouboglo G, Matar M, Moghnieh R and Daoud Z. Characterization and susceptibility of non-albicans Candida isolated from various clinical specimens in Lebanese hospitals. Front Public Health. 2023;11:1115055.

Kothalawala M, Jayaweera JA, Arunan S and Jayathilake A. The emergence of nonalbicans candidemia andevaluation of HiChrome Candida differential agar and VITEK2 YST® platform for differentiation of Candida bloodstream isolates in teaching hospital Kandy, Sri Lanka. BMC Microbiology. 2019;19(1):136.

Tan TY, Hsu LY, Alejandria MM, Chaiwarith R, Chinniah T, Chayakulkeeree M, Choudhury S, Chen YH, Shin JH, Kiratisin P, Mendoza M, Prabhu K, Supparatpinyo K, Tan AL, Phan XT, Tran TT, Nguyen GB, Doan MP, Huynh VA, Nguyen SM, Tran TB, Van Pham H. Antifungal susceptibility of invasive Candida bloodstream isolates from the Asia-Pacific region. Medical Mycology. 2016;54(5):471-477.

Ghrenassia E, Mokart D, Mayaux J, Demoule A, Rezine I, Kerhuel L, Calvet L, De Jong A, Azoulay E and Darmon M. Candidemia in critically ill immunocompromised patients: Report of a retrospective multicenter cohort study. Annals of Intensive Care. 2019;9(1):62.

Deorukhkar SC, Saini S, Mathew S. Virulence factors contributing to pathogenicity of Candida tropicalis and its antifungal susceptibility profile. International Journal of Microbiology. 2014;2014:456878.

Badiee P, Hashemizadeh Z. Opportunistic invasive fungal infections: Diagnosis & clinical management. Indian Journal of Medical Research. 2014;139(2):195-204.

Pandey N, Gupta MK, Paul P, Tilak R. Necessity to identify candida species accurately with minimum inhibitory concentration determination in each case of bloodstream infections. Journal of Infection and Public Health. 2020; 13(5): 753-858.

Barnes RA. Early diagnosis of fungal infection in immunocompromised patients. Journal of Antimicrobial Chemotheraphy. 2008;61(suppl_1):i3-i6.

Kaur R, Jaggi S, Dhakad MS and Rawat D. An etiological and antifungal profile of candidemia in children. Internation Journal of Community Medicine and Public Health. 2019;6(9):3899-3904.