The Effect of Vitamin E Supplement on Creatine Kinase MB, C-Reactive Protein, Creatinine and Urea Levels of Albino Rats Fed with High Fat Diet
Waribo, Helen Anthony *
Department of Medical Laboratory Science, Rivers State University, Nkpolu Oroworukwu, Port Harcourt, Rivers State, Nigeria.
Nkwazema, Amobi Kenneth
Department of Medical Laboratory Science, Pamo University of Medical Sciences, Tap Road, Elelenwo, Rivers State, Nigeria.
Aleruchi-Didia, Tarila Ngowari
Department of Medical Laboratory Science, Rivers State University, Nkpolu Oroworukwu, Port Harcourt, Rivers State, Nigeria.
Brisibe, Ngozi
Department of Medical Laboratory Science, Rivers State University, Nkpolu Oroworukwu, Port Harcourt, Rivers State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study was designed to assess the effect of vitamin E supplement on Creatine Kinase (CK-MB) C - reactive protein, (C-RP), urea and creatinine of albino rats fed with high fat diet.
Methodology: A total of twenty (20) albino rats of both sexes were used in this study. They were grouped into four (4) groups comprising of five (5) rats each. Group 1 was fed with normal diet; group 2 with normal diet and treated with vitamin E supplement (70 mg/kg); group 3 was fed with high fat diet only, while group 4 was fed with high fat diet and treated with vitamin E supplement (70 mg/kg). The treatments were administered daily using oral gavage method for a period 21 days. After the experimental period, the animals were sacrificed and blood samples collected for the analysis of CK-MB, CRP, urea and creatinine respectively using ELISA and colorimetric method with a spectrophotometer. Data generated were analyzed statistically using SPSS version 20 and P = 0.05 was considered statistically significant.
Results: The results revealed a significance difference in C-RP (mg/l) levels (P < 0.001) amongst the groups with a decreased C-RP levels in group 2 (1.43 ± 0.39) compared to group 1 (1.94 ± 0.53), then in group 4 (2.27 ± 0.53) which is also decreased compared to group 3 (3.31 ± 0.63). For CK-MB there was also a significant difference among the different groups. In group 2, it was 1.94 ± 0.65 ng/ml which was decreased compared to group 1 (2.22 ± 0.55), and group 4 (2.74 ± 0.68) compared to group 3 (3.31 ± 0.62). For the urea and creatinine results, there was no significant difference (p > 0.05) among the groups when the mean levels were compared.
Conclusion: This shows that administration of vitamin E to rats fed normal or high fat diet could significantly reduce the plasma levels of CRP and CK-MB hence having some ameliorative effects on high fat induced inflammatory and cardiovascular risks or conditions.
Keywords: Vitamin E supplement, creatine kinase-MB.C - reactive protein, urea, creatinine, high fat diet, albino rats