Anti-Inflammatory Activity Evaluation of the Extract and Topical Pre-Formulation of Morus nigra Fruits

Isabel Patricia Rojas

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina.

María Elizabeth Balderrama Coca

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina.

Josefina Villagra

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina.

Gabriela Del Carmen Bejarano

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina.

Nancy Roxana Vera

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina.

Marcos Adrián Reynoso *

Instituto de Estudios Farmacológicos Dr. Sampietro, Cátedra de Farmacoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, San Miguel de Tucumán, CP 4000, Argentina and Instituto de Biología Dr. Francisco Barbieri, Cátedra de Farmacodinamia, Facultad de Bioquímica, Química y Farmacia Universidad Nacional de Tucumán, Argentina.

*Author to whom correspondence should be addressed.


Abstract

Natural products are often a source for bioactive compounds which have great potential for developing novel therapeutic agents. In this sense, the present study aimed to formulate and evaluate a gel containing Morus nigra leaf extract and to evaluate its anti-inflammatory (in-vivo) and antioxidant (in-vitro) activity. The anti-inflammatory activity was evaluated using Carrageenan-induced paw oedema. The antioxidant activity is evaluated by the lipid peroxidation inhibition and DPPH radical scavenging method. The results indicated that the extract (oral and topical route) and the blackberry gel (topical route) produced a significant anti-inflammatory activity (p < 0.05) compared to the untreated control, and similar to the reference drugs (ibuprofen and diclofenac). Regarding antioxidant activity, the Morus nigra extract also showed a significant activity in the tested models. Based on the above observations and results, mora gel formulation was found to be a promising anti-inflammatory formulation and further extensive in-vitro and in-vivo studies are warranted to evaluate its safety and biological potency and which may be useful for further clinical applications.

Keywords: Anti-inflammatory activity, mora gel, Morus nigra, blackberry, preformulation


How to Cite

Rojas, I. P., Coca, M. E. B., Villagra , J., Bejarano , G. D. C., Vera , N. R. and Reynoso , M. A. (2023) “Anti-Inflammatory Activity Evaluation of the Extract and Topical Pre-Formulation of Morus nigra Fruits”, Journal of Pharmaceutical Research International, 35(5), pp. 1–12. doi: 10.9734/jpri/2023/v35i57324.

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References

García L, Rojo D, Gómez L, Hernández M. Plantas con propiedades antiinflamatorias. Revista Cubana de Investigaciones Biomédicas. 2002;21(3):214-6.

Quiñones M, Miguel M, Aleixandre A. Los polifenoles, compuestos de origen natural con efectos saludables sobre el sistema cardiovascular. Nutrición Hospitalaria 2012;27(1):76-89.

Ismail HF, Hashim Z, Soon WT, Rahman NSA, Zainudin AN, Majid FAA. Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo. J. of Traditional Complementary Med. 2017;1-14.

Griffiths K, Aggarwal BB, Singh RB, Buttar HS, Wilson D, DeMeester F. Food Antioxidants and Their Anti-Inflammatory Properties: A Potential Role in Cardiovascular Diseases and Cancer Prevention. Diseases. 2016;4(3):28.

Yiğit, D., Mavi, A., Aktaş, M. Antioxidant activities of black mulberry (Morus nigra). EÜFBED - Fen Bilimleri Enstitüsü Dergisi Cilt-Sayı. 2008;1-2.

Gerasopoulos, D, Stavorulakis, G. Quality characteristics of four mulberry (Morus sp.) cultivars in the area of China. J. Sci. Food Agric. 1997;73: 261–264.

Baytop, T. Türkiyede bitkilerle tedavi. İstanbul Eczacılık Fakültesi Yayınları, İstanbul. 1999;444 (In Türkish).

Naderi GA, Asgary S, Sarraf-Zadegan N, Oroojy H, Afshin-Nia F. Antioxidant activity of three extracts of Morus nigra. Phytother Res. 2004;18(5):365-9.

Ody Mnimh P. The Complete Guide Medicinal Herbal, 2nd edn.: Dorling Kindersley, London. 2000 92–93.

Garzon G.A. Anthocyanins As Natural Colourants And Bioactive Compounds. A Review. Acta biol. Colomb 2008;13(3): 27 - 36.

Mesquita Padilha M, Cardoso Vilela F, Dias da Silva M, dos Santos M, Alves-da-Silva G and Giusti-Paiva A. Antinociceptive Effect of the Extract of Morus nigra Leaves in Mice. J. Med. Food. 2009;12(6): 1381–1385.

Hua-Bin L, Ka-Wing C, Chi-Chun W, King-Wai F, Feng C, Yue J. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry 2007; 102:771–776.

Po-Yuan C, Jhih-Ying C, Li-Chun H. Antioxidant Activity of Phenolic Compounds Extracted from Fresh and Dried Water Caltrop Pulp (Trapa taiwanensis Nakai). Journal of Food and Drug Analysis. 2008;16(3):66-73.

Majumdar S, Dave R. Formulation study of gel containing Pterocarpus santalinus extract for its anti-inflammatory activity. World Journal of Pharmacy and Pharmaceutical Science. 2013;2(6):4951–64.

Khullar R, Kumar D, Seth N, Saini S. Formulation and evaluation of mefenamic acid emulgel. Saudi Pharm. 2012; 20:63-7.

Nardi-Ricart A, Linares MJ, Villca-Pozo F, Pérez-Lozano P, Suñé-Negre JM, Bachs-deMiquel L, et al. A new design for the review and appraisal of semi-solid dosage forms: Semi-solid Control Diagram (SSCD). PLoS ONE. 2018;13(9).

Procedimiento normalizado de trabajo. Determinación de la extensibilidad. Formulario Nacional 1º edición, 2003. PN/L/FF/003/00. Orden SCO/3262/2003. BOE Nº 283. España.

Reynoso, M, Vera, N, Aristimuño, ME, Daud, A., Riera, AS Antinociceptive activity of fruits extracts and “arrope” of Geoffroea decorticans (chañar). J. Ethnopharmacology. 2013;145:355–362.

Sun T, Ho CT. Antioxidant activities of buckwheat extracts. Food Chemistry. 2005;90:743-749.

Winter CA, Risley EA, Nuss GW. Carrageenan-induced edemas in hind paw of the rats as an assay of anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine 1962;3:544–547.

García MD, Fernández MA, Sáenz MT, Ahumada MC. Antiinflammatory effects of different extracts and harpagoside isolated from Scrophularia frutescens. ll Farmaco. 1995;51:443–6.

Chalons P, Amor S, Courtaut F, Cantos-Villar E, Richard T, Auger C, Chabert P, Schni-Kerth V, Aires V, Delmas D. Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages. Nutrients. 2018;10:1856.

Schaible HG, Ebersberger A, Banchet GS. Mechanisms of pain in arthritis. Ann. N. Y. Acad. Sci. 2002;966:343–354.

Woolf C, Allchorne A, Safieh‐Garabedian B, Poole S. Cytokines, nerve growth factor and inflammatory hyperalgesia: The contribution of tumour necrosis factor α. Br. J. Pharmacol. 1997;121:417–424.

Hernandez PM, Rabanal Gallego R. Evaluation of the anti- - inflammatory and analgesic activity of Sideritis anariensis var. pannosa in mice. J. Ethnopharmacol 2002;81:43-47.

Chen H, Pu J, Liu D, Yu W, Shao Y, Yang G, Xiang Z, He N. Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.) PLoS ONE. 2016;11:e0153080.

Murray A, Kisin E, Castranova V, Kommineni C, Gunther M, Shvedova A. Phenol-induced in vivo oxidative stress in skin: Evidence for enhanced free radical generation, thiol oxidation, and antioxidant depletion. Chem. Res. Toxicol. 2007;20:1769-77.

Huang, MH, Huang SS, Wang BS, Wu CH, Sheu MJ, Hou WC, Lin SS, Huang GJ. Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. J. Ethnopharmacol. 2011;133: 743–750.

Saxena M., Saxena J., Pradhan A. Flavonoids and phenolic acids as antioxidants in plants and human health. Int. J. Pharm. Sci. Rev. Res. 2012;16: 130–134.

Özgen M., Serçe S., Kaya C. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus alba fruits. Sci. Hortic. (Amsterdam). 2009; 119:275–279.

Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol. Nutr. Food Res. 2007;51:675–683.

Sung Ho Lim and Chang-Ik Choi. Pharmacological Properties of Morus nigra L. (Black Mulberry) as a pomising nutraceutical resource. Nutrients. 2019; 11(2):437.