DNA Extraction and Quantification of Some Economically Important Fruits in Tamil Nadu
K. Kamalakkannan
Department of Biotechnology, Nandha College of Pharmacy, Erode 638052, Tamil Nadu, India.
V. Balakrishnan
Department of Botany, Arignar Anna Government Arts College, Namakkal 637002, Tamil Nadu, India.
R. Sumathi
Department of Biotechnology, Nandha College of Pharmacy, Erode 638052, Tamil Nadu, India.
N. Manikandaprabu
Department of ECE, Nandha Engineering College, Erode 638052, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Humans can benefited greatly from functional foods in order to preserve good health. The research began with the extraction of DNA from the white edible section of Cocos nucifera and the flesh of Borassus flabellifer fruits using the simple, Edward, PCI, and CTAB methods. To confirm the existence of DNA, the complete isolated DNA was examined using the DPA and Keller-killani tests. Following the identification test, UV spectroscopy was used to quantify the results by taking the absorbance of the samples at 260nm and 280nm and quantified using absorbance ratio procedure. When compared to other procedures, the CTAB approach produces acceptable results at low temperatures. Both the white edible part of Cocos nucifera and flesh of Borassus flabellifer gave high purity DNA on extraction. Further the concentration of DNA obtained from Cocos nucifera was 4.94, 4.7, 5.25 and 8.25 µg\ml by simple, Edward PCI and CTAB methods respectively and for Borassus flabellifer the concentration DNA isolated was found to be 2.48, 2.14, 4.25, 6.4 µg\ml by simple, Edward PCI and CTAB methods respectively.
Keywords: Borassus flabellifer, cocos nucifera, DNA isolation, electrophoresis