Cytotoxic Activity of Zinc Oxide Nanoparticle Synthesis Using Leaves Extract of Abies webbiana
M. Srinisha
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
M. Jeevitha *
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
S. Rajeshkumar
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Selvaraj Jayaraman
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study aimed to analyse the cytotoxicity of zinc oxide nanoparticles (ZnO NPs) that are biosynthesized using extract of Abies webbiana
Methodology: Zinc oxide nanoparticles were synthesized by green approach using A. webbiana and were characterized by UV- vis spectroscopy. The powdered A. webbiana mediated ZnO NPs were subjected to cytotoxic tests for different concentrations by brine shrimp lethality assay.
Results: ZnO NPs synthesized from A. webbiana showed reduced toxicity at lower concentrations. After 48 hours, it was found that at a minimal concentration of 5 µl, 90% of the nauplii and in concentration of 10 µl and 20 µl, 80% of the nauplii were alive. At a concentration of 40 µl and 80 µl, 70% of the nauplii were alive. Whereas the control showed 100% of the nauplii to be alive.
Conclusion: Various nanoformulations of A. webbiana mediated ZnO NPs can be developed at optimal concentrations for therapeutics that are safe and economical.
Keywords: Abies webbiana, brine shrimp lethality assay, cytotoxicity, green synthesis, innovative technology, zinc oxide nanoparticles