Anticancer Potential of A. marmelos Fruit Extract in Human Colon Cancer Cell Lines is Mediated through the Regulation of EMT Signalling Molecules
Sheron Blessy
Saveetha Dental college, Saveetha Institute of Medical and Technical Science (SIMATS), Saveetha University, Chennai, India.
R. Gayatri Devi *
Department of Physiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science (SIMATS), Saveetha University, Chennai-600077, India.
J. Selvaraj
Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Science (SIMATS), Saveetha University, Chennai-600077, India.
A. Jothi Priya
Department of Physiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science (SIMATS), Saveetha University, Chennai-600077, India.
*Author to whom correspondence should be addressed.
Abstract
Background and Aim: A.marmelos is commonly called as Bael and is used as a medicinal plant mostly in the Ayurveda. Bael has the property of gastrointestinal effect. Bael phytochemicals are radioprotective, chemoprotective and it has efficacious properties in the treatment of cancer and its preventions. The main aim of this study is to estimate an anticancer activity of Bael in colon cancer cells of human.
Materials and Methods: Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Phosphate-buffered saline(PBS) are used as materials. The cell line used is HT 29. MTT test was used to check cell viability by calorimetric technique. The HT 29 cell was exposed to different mediums. Then the cell viability is calculated. Real time PCR was used to analysis gene expression in which cDNA synthesis was performed in varied sample volume. Melting curves were acquired for all samples. Data is analyzed by comparative CT method. It was analyzed by graph pad prism.
Results: The findings showed that the effect of Bael fruit extraction of HT 29 cells shows a significance of P<0.05. One of the parameters is treated with untreated control cells. The other two parameters are 100μg, 200μg. In Vimentin mRNA and E cadherin mRNA expression one is treated with untreated control cells and 100μg.
Conclusion: This concludes that the properties of the bael can act as an anticancer potential in human colon cancer cell HT 29 through EMT signalling molecules.
Keywords: Bael, anticancer, HT 29 cells, EMT signalling, innovative techniques