Effect of Methotrexate and Linoleic Acid on BAX/BCL2 Ratio in Human Hepatocyte Cell
Ayse Gül Kabakci *
Department of Anatomy, Faculty of Medicine, Cukurova University, Adana, Turkey.
Halil Mahir Kaplan
Department of Medical Pharmacology, Faculty of Medicine, Cukurova University, Turkey.
Ergin Şingirik
Department of Medical Pharmacology, Faculty of Medicine, Cukurova University, Turkey.
Memduha Gülhal Bozkır
Department of Anatomy, Faculty of Medicine, Cukurova University, Adana, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Aims: To investigate the effect of methotrexate and linoleic acid on BAX/BCL 2 ratio in human hepatocyte cell.
Study Design: Original Research Article.
Place and Duration of Study: The study was carried out in partnership with the Department of Anatomy and Department of Medical Pharmacology of Çukurova University Faculty of Medicine, using the laboratory facilities of the Department of Medical Pharmacology.
Methodology: Human hepatocyte cell line (CRL-11233) cells obtained from the American Type Culture Collection Organization (ATCC) were used. Expressions of apoptotic pathway markers, apoptosis inducing factor BAX, BCL2 and BAX/BCL2 were evaluated. All analyzes were examined in four groups (Group 1; control, Group 2; linoleic acid given, Group 3; methotrexate given and Group 4; linoleic acid and methotrexate given).
Results: The mean ± standard error values of the obtained results as nanogram / milliliter (ng / ml) are in Group I, Group II, Group III and Group IV, respectively; BAX values, 0.900 ± 0.1864, 1.002 ± 0.2098, 8.352 ± 1.467 and 4.295 ± 1.522, BCL 2 values, 13.93 ± 1.198, 13.92 ± 1.739, 2.938 ± 1.059 and 9.250 ± 1.492 and BAX/BCL2 values 0.065, 0.072, 2.843 and 0.464.
Conclusion: While BAX/BCL2 level increased in the group given methotrexate, it decreased in the group given linoleic acid and methotrexate.
Keywords: BAX/BCL2, hepatocyte, liver, linoleic acid, methotrexate