Melissa officinalis L. Essential Oil: Chemical Composition, Antioxidant, Antibacterial and Antifungal Activities- in vitro Study
Hafedh Hajlaoui
Research Unit Valorization and Optimization of Resource Exploitation (UR16ES04), Faculty of Science and Technology of Sidi Bouzid, University of Kairouan, Campus University Agricultural City - Sidi Bouzid 9100, Tunisia.
Soumaya Arraouadi
Regional Center of Agricultural Research (CRRA) Sidi Bouzid, Gafsa Road Km 6, PB 357, Sidi Bouzid 9100, Tunisia and Laboratory of Valorization of Unconventional Waters, INRGREF, University of Carthage, Tunisia.
Emira Noumi
Department of Biology, Hail University, College of Science, P.O.Box 2440, 81451 Ha’il, Kingdom of Saudi Arabia and Laboratory of Bioressources, Integrative Biology and Recovery, High Institute of Biotechnology-University of Monastir, Monastir 5000, Tunisia.
Mejdi Snoussi *
Department of Biology, Hail University, College of Science, P.O.Box 2440, 81451 Ha’il, Kingdom of Saudi Arabia and Laboratory of Genetic, Biodiversity and Valorization of Bioressources, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Taher Hadded BP 74, 5000 Monastir, Tunisia.
Adel Kadri
Faculty of Science and Arts in Baljurashi, Albaha University, P.O.Box 1988, Albaha, Saudi Arabia and Faculty of Science of Sfax, Department of Chemistry, University of Sfax, B.P. 1171, 3000, Sfax, Tunisia.
*Author to whom correspondence should be addressed.
Abstract
The present investigated chemical composition of Melissa officinalis L. essential oil (MOEO) extracted by hydrodistillation. The MOEO was analyzed by gas chromatography-mass spectrometry (GC-MS), revealing the presence of thirty compounds, representing 98.46% of the oil constituents. The predominant components were 1,8-cineole (39.80%) followed by citronellol (16.66%), geraniol (12.25%), myrcene (5.85%) and geranial (5.45%). The antioxidant potential of MOEO has been summarized using DPPH test (IC50), superoxide anion (O2−·) scavenging activity (IC50), β-carotene (IC50) and reducing power (FRAP) (EC50). Results demonstrate strong scavenging superoxide anion capacity and moderate to weaker activity against the other assays. Potent inhibitory effect has been observed towards Micrococcus luteus and Bacillus cereus as well as the Candida albicans ATCC 90028, C. tropicalis (Strain 1) and C. albicans (Strain 8). Our work provides a view for the further studies on the antioxidant and antimicrobial of the MOEO and its main components.
Keywords: Melissa officinalis L, essential oil, GS-MS, antioxidant, antibacterial, antifungal