Calculation of the Zone of Inhibition and Sensitivity Pattern of Fenugreek Seeds Extract and Negila sativa Seeds Extract on ESBL E. coli Growth Plates
Ata Ur Rehman *
Department of Pharmacology, Hamdard College of Medicine and Dentistr, Hamdard University Karachi, Pakistan.
Kiran Khan
Department of Community Health Sciences, Iqra Medical and Dental College, Iqra University Karach, Pakistan.
Ghazala Nasim Pasha
Department of Community Health Sciences, United medical and dental College, Karachi, Pakistan.
Ulfat Sultana
Department of Pharmacology, Muhammad Medical College Peshawar, Pakistan.
Tajwar Sultana
Department of Pharmacology, Muhammad Medical College Peshawar, Pakistan.
Ruqaya Nangreja
Department of Pathology, Baqai medical University, Karchi, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Background: The reason behind the poor outcomes in treating bacterial infections is mainly the limited treatment options. The strains of ESBL are generally resistant to broad-spectrum penicillin, monobactams, and third-generation cephalosporins. Literature is currently highlighting the efficacy of herbs against many resistant organisms. Hence, the study aims to identify the sensitivity pattern and zone of inhibition of Fenugreek seed extract and Nigella sativa Extract against ESBL E. Coli.
Methodology: The calculated sample size was n = 40. The ESBL E. coli growth plate samples were recruited from an associated lab which were identified by performing biochemical tests on appropriate media i.e. Mueller-Hinton agar was used. Agar dilution methods were performed to examine the antibacterial effects of fenugreek seed extract (FGSE) and Nigella sativa seed extract (NGSE) against ESBL E. coli. wells of 8mm diameter were punched in the inoculated plates by using sterile cork borer, 100μl of different concentrations of Fenugreek seed extract (30, 40, 50, 60, and 80mg/ml) and Nigella sativa extract (30, 40, 50, 60, and 80mg/ml) were added to wells and the plates and the plates were incubated for 24 to 48 hours at 37°C. After 48 hours presence or absence of zones of inhibition were investigated.
Results: Total 40 samples were calculated for each concentration of the solution of extracts i.e. FGSE group and NGSE group. The effective concentration of FGSE was found to be 40mg/ml and for NGSE was found to be 60mg/ml that showed maximum. Both the extracts showed inhibition of growth of organism at different concentrations table 2 shows the zone of inhibition.
Conclusion: The Fenugreek seed extract at 40mg/ml and Nigella sativa seed extract at 60mg/ml have highest sensitivity and are effective in inhibiting the growth of ESBL E. coli on growth plates.
Keywords: Fenugreek seed, Nigella sativa, sensitivity, ESBL E. coli