Study on the Plasmid and Genomic DNA Stability of DH5α Host System
Laxmisai Dixitha Vengili
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Phavethra Sago
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Yaswanth Kumar Reddy Kuncha
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Venkata Suneela Kolli
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Lasya Sri Varugu
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Sree Lakshmi Mandadapu
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
Suryanarayana Veeravilli
Department of Humanities and Basic Sciences, Aditya Engineering College, Kakinada, Andhra Pradesh, India.
Praveen Kumar Vemuri *
Department of Biotechnology, Koneru Lakshmaih Education Foundation, Guntur, Andhra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media.
Keywords: DH5α, DNA, EcoRI, HindIII, BamH1, plasmid