Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV
Narges Chamkouri *
Abadan School of Medical Sciences, Abadan, Iran.
Mona Vafaeizadeh
Student Research Committee, Abadan School of Medical Sciences, Abadan, Iran.
Ayyub Mojaddami
Department of Medicinal Chemistry, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Seyyed Abed Hosseini
Abadan School of Medical Sciences, Abadan, Iran.
Tahereh Momeni Isfahani
Department of Chemistry, Islamic Azad University, Arak Branch, Iran.
Aida Ghorbani Golkhajeh
Science and Research Branch, Islamic Azad University, Tehran, Iran.
Milad Janghorban Lariche
Abadan School of Medical Sciences, Abadan, Iran.
Seyed Mohammad Ali Malaekeh
Abadan School of Medical Sciences, Abadan, Iran.
*Author to whom correspondence should be addressed.
Abstract
In this work, solid phase extraction (SPE) was proposed for simultaneous determination of trace levels of vitamin B6, B9 and B12. High performance liquid chromatography with UV detection (HPLC-UV) was used. The experimental conditions including pH of sample and type and volume of extraction solvent were studied by one-factor-at-a time method. The limit of detection in the optimal conditions was 14.91, 10.03, and 7.25 ngkg-1 for vitamins B6, B9 and B12 respectively and relative standard deviations (RSD) of the analysis less than 8% (n= 3). Recoveries of vitamins B6, B9 and B12 were in the ranges of 80–93%. The proposed method was successfully applied for the simultaneous determination of vitamin B6, B9 and B12 in halophyte samples such as, Salicornia, Suaeda monoica, Suaeda aegyptiaca, Sueada vera and Sueada martima.
Keywords: Vitamins B, halophyte, HPLC-UV.