Cardio Protective Role of Novel Gemmo Therapeutically Treated Glycyrrhizaglabra against Isoproterenol Induced Myocardial Injury
Amees Akhter
Primary and Secondary Health Care Department, Punjab, Pakistan.
Munazzah Meraj *
Biochemistry Department (IPRS), Peoples University of Medical and Health Sciences for Women (PUMHSW), Nawabshah, Pakistan.
Mubeena Laghari
Department of Biochemistry, Liaquat University of Medical and Health Sciences (LUMHS), Pakistan.
Rao Irfan
IPS, PUMHSW, Nawabshah, Pakistan.
. Fouzia
Secondary School Teacher Science, School Education Department, Punjab, Pakistan.
Gulam Zahara Jahngir
Center of Applied Molecular Biology (CAMB), University of Punjab, Lahore, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Aim: To explore cardio protective potential of Glycyrrhiza glabraas against chemically induced myocardial injury in experimental animals by using their blood (serum) for subsequent determination of enzyme levels.
Methodology: Glycyrrhizaglabra with known cardio protective potential was used against chemically induced myocardial injury in experimental animals. These animals were divided into seven groups having ten animals each. Seventy rabbits of both sexes, 1-1.5 kg weight was included while pregnant rabbits weight >1.5 kg were excluded. Glycyrrhiza glabraleaves after gemmo-therapeutic treatment were subjected for phytochemical screening. The blood was drawn from the neck region; serum was separated for biochemical analysis. Pretreatment with native and gemmo-extract resisted to rise (P≤0.05) cardiac enzymes (CK-MB, SGOT, sGPT, LDH).
Results: Phytochemical screening for alkaloids 7% & 7.5%, flavonoids 21% & 24%, glycosides 16% and 20%, tannic acid10% & 15.5%, and saponins 8% & 15% in gemmo and native extract respectively.
Conclusion: It was studied that in isoproterenol injured rabbits, gemmo-extract was significantly better (P≤0.05) than native-extract in curative as well as in preventive treatment to normalizing serum cardiac enzymes.
Keywords: Cardiovascular diseases, cardiac enzymes, CK-MB, Glycyrrhiza glabra, Isoproterenol, LDH, SGOT, SGPT