In-vitro Antioxidant, Antidiabetic Activities and Phytotoxic Profile of Alkanna tinctoria (Boraginaceae)
Rahmat Ali Khan *
Department of Biotechnology, UST Bannu, KPK, Pakistan.
Aamir Khan Awan
Department of Biotechnology, UST Bannu, KPK, Pakistan.
Mushtaq Ahmed
Department of Biotechnology, UST Bannu, KPK, Pakistan.
Huda Alkreathy
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Alia Gul
Department of Botany, Hazara University Mansehra, KPK, Pakistan.
Amir Muhammad Khan
Department of Botany, University of Mian Wali, Pakistan.
Arshad Mahmood
Unit Soil Science and Plant Nutrients, Brunei Agricultural Research Center, Brunei Darussalam.
Asif Khan
Department of Biotechnology, UST Bannu, KPK, Pakistan.
Muhammad Nisar Ul Haq
Department of Botany, Hazara University Mansehra, KPK, Pakistan.
Khalid Saleh Alzahrani
Ministry of health King Faisal medical complex, Taif Medical resident, Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
Plants are the most important source of biological compounds that are used to achieve biological activity. The originating of crude extracts of plants is broadly used as therapeutic drugs to treat various infectious damages. The present study was arranged to investigate the potential of Alkanna tinctoria Tausch, roots extract, collected from district Bannu, Pakistan in contradiction of various human pathogenic infections, resistant and antioxidant activities like DPPH (2, 2- diphenyl-1-picrylhydrazy) free radicals scavenging, hydrogen peroxide scavenging, ABTS [2, 2-azinobis (3- ethylbenzthiazoline-6-sulphonic acid)] scavenging activity and phytotoxicity effect. The current study was focused on the phytochemical investigation and pharmacological activities of methanolic extract of Alkanna tinctoria. The plant extracts were examined for antioxidant scavenging assay by using DPPH & ABTS free radicals, Hydrogen peroxide, alpha amylase and Phytotoxicity. Ascorbic acid was taken as standard. Methanolic crude extract showed high phenolic contents and significant antioxidant activity (DPPH, H2O2 and ABTS). All the concentration of Alkanna tinctoria also showed phytotoxicity effect and was found to have significant antioxidant potential. From the findings of the present study it was concluded that the methanol extract of Alkanna tinctoria have various antioxidant, anti-diabetic and phytotoxic activities. Furthermore, analysis is needed to investigate its mechanism of pharmacological activities.
Keywords: Alkanna tinctoria, phytochemical investigation, pharmacological activities, DPPH, H2O2, ABTS, Phytotoxicity