The Significance of Nigella sativa on Histomorphological Variations of Seminiferous Tubules in Doxorubicin Treated Albino Rats
Ashok Kumar
Department of Anatomy, SMBBMC, Pakistan.
Anjum Rehman
Department of Gynae & Obs, SMBBMC, Pakistan.
Sadia Sundus *
Department of Anatomy, Iqra Medical & Dental, Karachi, Pakistan.
Saher Fatima
Department of Gynae & Obs, SMBBMC, Pakistan.
Zeeshan Ahmed
Department of Surgery, SMBBMC, Pakistan.
Asma Aijaz
Department of Anatomy, Karachi Medical & Dental College, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Objective: To analyze the impact of nigella sativa on Histomorphological changes in doxorubicin treated albino rats.
Research Design: Experimental research.
Abode of Study: Anatomy Department, BMSI, Karachi, Pakistan.
Materials and Methods: Piloted on 40 albino rats for 35 days in the Anatomy Department of BMSI. They were 3-4 months old and about 221 to 238 gm in weight. Rats were separated into 4 groups, Ao, Bo, Co & Do. Ao represented as control, Bo recieved Doxorubicin (DOX) 0.003g/1000g/ at 7TH day intraperitonealy, Co recieved Nigella sativa 1000mg/1000g everyday oral along with Doxorubicin 0.003g/1000g/ at 7TH day intraperitonealy and Do recieved Nigella sativa 1000mg/kg everyday oral. At the end of experiment, rats were dissected and conserved slides for staining.
Results: Bo showed loss of normal architecture of seminiferous tubules and diverse level of atrophy while condition was improved in Co which were given Doxorubicin with extract of Nigella sativa.
Conclusion: Our result tells us that Nigella sativa amended the histomorphological changes.
Keywords: NG (Nigella sativa), malondialdehyde (MDA), histomorphological, ROS (reactive oxygen species), plasmalemma