Lipid Charecterization and Fatty Acid Profiles of Clarius batrachus and Its Defense Activity
Giridharan Bupesh *
Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India and Department of Virology, King Institute of Preventive Medicine, Guindy, Chennai, Tamilnadu, India.
Chinnaiah Amutha
Department of Animal Behavior and Physiology, Madurai Kamaraj University, Madurai, Tamilnadu, India.
Krishnan Saravana Murali
Department of Virology, King Institute of Preventive Medicine, Guindy, Chennai, Tamilnadu, India.
Natarajan Saran
Department of Virology, King Institute of Preventive Medicine, Guindy, Chennai, Tamilnadu, India.
Pitchaipillai Shanthi
Department of Zoology, Nehru memorial college, Puthanampatti, Tamilnadu, India.
Sakthivel Vasanth
Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India.
E. Manikandan
Department of Physics, Thiruvalluvar University, Vellore, Tamilnadu, India.
Periasamy Subramanian
Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Innate Defense Components were being useful to identify and understand their mode of action on microbial pathogens. The present study portrays the qualitative and quantitative determination of endogenous lipids from the skin, intestine, kidney and liver of Clarius batrachus. The skin lipids were identified by TLC, which constitutes phosphatydyl serine (Ptd Ser) (48%), glycerophospholipids (23%), phosphoglyceride (20%), and 7% of cholesterol (C). The GC-MS FAME profile of the Skin comprised, the saturated fatty acid (SFA) with n-16 and n-18 as major fatty acids, 25.04% of palmitic (PAM) and 10.23% of stearic acid (STA). The fatty acid profiles and lipid classes of other organs were also identified and quantitated. The results from the present study bestowed to the skin lipid from the C.batrachus can be developed as a novel drug for multidrug resistant bacterial pathogens.
Keywords: GC-MS FAME analysis, thin layer chromatography (TLC), lipid classes, multidrug resistant pathogens, Clarius batrachus