Correlation of IDH1-(R132H) and ATRX Expression with the Histopathological Grades of Glial Tumours: A Study Protocol
Pooja Jha *
Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, India.
Samarth Shukla
Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, India.
Sunita Vagha
Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, India.
Ravindra P. Kadu
Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Brain tumors contribute significantly to morbidity as they can lead to neurological deficits, mental alterations and have a poor survival rate. The utmost frequent varieties of primary brain tumors are gliomas. Modern data on permeated gliomas showcased persistent modification within the involved genes in the chromatin remodeling routes such as α-thalassemia/mental-retardation syndrome-X-linked gene (ATRX) and IDH1. Till date, the authenticity of ATRX in predicting isocitrate dehydrogenase (IDH1) mutations in gliomas, is unclear. This study attempts to assess the correlation between IDH1 mutation and ATRX expression and histopathological grading in glial tumors.
Methods: This will be a retrospective analytical study conducted in Pathology Department, of JNMC, Wardha in coordination with the Department of Neurosurgery, AVBRH. This study will include histochemical analysis of 45-50 resected specimens of confirmed cases of Glial tumours. The correlation between IDH1 mutation and ATRX expression with the histopathological grades in glial tumors will be analysed in comparison with the present-day molecular advances.
Results: Significant correlation between IDH1 mutation and ATRX expression with the histopathological grades is expected.
Conclusion: Conclusion will be drawn on careful analysis of data.
Keywords: ATRX marker, IDH1-(R132H), mutation, grading, glioma, glial tumours