Anti-proliferative Effect of Isoflavones Isolated from Soybean and Soymilk Powder on Lymphoma (DG 75) and Leukemia (CEM) Cell Lines
C. Apprey
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana.
C. Larbie *
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana.
F. K. N. Arthur
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana.
R. Appiah-Opong
Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana.
R. A. Annan
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana.
I. Tuffour
Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana.
*Author to whom correspondence should be addressed.
Abstract
Aim: Isoflavones in soybean have been linked to reduced risk of some cardiovascular diseases as well as some type of cancers. Extensive epidemiological, in-vitro and animal data suggests lower incidence of cardiovascular disease, hormone-dependent cancers of the breast and prostate, for consumers of soy and soy products. In this study, isoflavones were isolated from Soybean (SB) and soymilk powder (SMP) and the anti-proliferative effects of these isoflavones on Burkitt’s lymphoma (DG-75) and Leukemia (CEM) cell lines were investigated.
Place of Study: Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana between June and December, 2014.
Methodology: Isoflavanoids were isolated and HPLC done to confirm the isolate. Isoflavanoids were isolated form SB and SMP and the anti-proliferative effect of isoflavanoid isolated from SB, SMP, pure genistein and daidzein on lymphoma and leukaemia cells were compared using tetrazolium-based colorimetric (MTT) assay.
Results: The inhibitory effect of genistein on leukemia (CEM) cell line was stronger (IC50 = 28.4 µM) than daidzein (IC50 = >100 µM). For the DG 75, genistein had a better effect (IC50 = 11.05 µM than daidzein (IC50> 100 µM). The IC50 for SMP were 193.92 µg/ml (DG-75) and 54.17 µg/ml (CEM). In terms of selectivity index (SI), genistein had the highest (>6.95) on CEM followed by genistein (>3.52) and then SB (>1.16).
Conclusion: SMP had a better anti-proliferative effect on both CEM and DG-75 compared with SB.
Keywords: Soybean, anti-proliferative, daidzein, genistein