Effect of Kabasura Kudineer Extract on Inhibitory Kappa B Kinase Beta and m TOR mRNA Complex Expression in Lung Cancer Cells (A549 Cell)
S. P. Ashwin
Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, India.
S. Preetha *
Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, India.
J. Selvaraj
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, India.
G. Sridevi
Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Lung cancer has the highest mortality rate and highest rate of metastasis. Kabasura kudineer was used widely during the Covid period. The aim of the study is to find the effect of kabasura kudineer extract on inhibitory kappa B kinase beta and mTOR mRNA complex expression in lung cancer cells.
Materials and Methods: Cell viability test was done using MTT assay. mRNA expression of inhibitory kappa B Kinase and mTOR was done by real-time PCR. The data was analysed statistically by one way analysis of variance and Duncan multiple range test with graph prism version 5. p<0.05 was considered significant.
Results: Kabasura kudineer caused a marked increase in cell death in dose dependent manner. At the end of 48 hours, maximum inhibition was at 300 and 400 µg/ml. Kabasura kudineer has inhibited the mRNA expression of inhibitory kappa B Kinase and mTOR in lung cancer cell lines (A549 cell).
Conclusion: The study indicates that kabasura kudineer extract has anti cancer activity on the lung cancer cell line (A549 cell).
Keywords: Kabasura kudineer, lung cancer, inhibitory kappa B kinase, mTOR, novel method