Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Tacrolimus in Bulk and Pharmaceutical Nanoparticles
Snehal Patel *
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Chintan Aundhia
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Avinash Seth
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Nirmal Shah
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Dipti Gohil
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Ghanshyam Parmar
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Sapna Desai
Pioneer Pharmacy Degree College, Vadodara, Gujarat, India.
Kartik Pandya
Department of Pharmacy, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
*Author to whom correspondence should be addressed.
Abstract
A UV-visible spectrophotometric method for quantifying tacrolimus in nanoparticles has been developed and validated, and it is accurate, simple, reproducible, and affordable. For drug analysis, optimal conditions have been identified. 291 nm was discovered to be the maximum wavelength (max). In the range of 0.2-1.8 mg/ml, the response was linear (r2 = 0.9989). The intra- and inter-day relative standard deviations for precision studies were found to be less than 2%, indicating that the procedure is precise.
Keywords: Tacrolimus, UV visible spectrometry, validation, ICH