In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia
Rajendran Raja Priya *
Department of Chemistry, Government Arts College (Autonomous), Salem-636007, India.
N. Bhadusha
Department of Chemistry, Government Arts College (Autonomous), Salem-636007, India.
Veramuthu Manivannan
Department of Chemistry, Government Arts College (Autonomous), Salem-636007, India.
Thanthoni Gunasekaran
Department of Chemistry, Government Arts College (Autonomous), Salem-636007, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia.
Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined.
Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml.
Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.
Keywords: H. arifolia, qualitative phytochemical activity, quantitative phytochemical activity, free radicals, polyphenol, antioxidant activity