In vitro Anticancer Activity of Hibiscus vitifolius Flowers Ethyl Acetate Fraction against Hepg2 Cell Line
D. Prabhakaran *
Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India and Chettinad Cement Corporation Ltd., Ariyalur, Tamil Nadu, India.
A. Rajeshkanna
Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
M. M. Senthamilselvi
Government Arts College, Kulithalai, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: The roots of Hibiscus vitifolius (Malavaceae) are used for the treatment of jaundice in the folklore system of medicine in India. This study is an attempt to evaluate anticancer activity of the flowers of Hibiscus vitifolius against Liver Cancer cell Line (HepG2).
Materials and Methods: In vitro cytotoxicity activity was carried out to screen cytotoxicity potency of the ethyl acetate fraction from Hibiscus vitifolius flower extract at different concentrations against HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is predictable to confirm the cytotoxicity.
Results: The ethyl acetate fraction from the flower extract of Hibiscus vitifolius was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 150 µg/ml against Liver Cancer HepG2 cell line.
Conclusion: These findings suggest that the ethyl acetate fraction obtained from the flower EtOH extracts of Hibiscus vitifolius have the potent anticancer activity and thereby justifying its ethnopharmacological claim.
Keywords: Hibiscus vitifolius, anti-cancer activity, MTT assay, HepG2, cytotoxicity.