Cytotoxic Effect of Ethanol Extract of Abrus precatorius Leaves on Raw 264.7 and SK-N-SH Cell lines
Krishnaprabha Naduchamy
Immunology Laboratory, Department of Pharmacy, Annamalai University, Annamalai Nagar- 608002, Tamilnadu, India.
Varadarajan Parthasarathy *
Immunology Laboratory, Department of Pharmacy, Annamalai University, Annamalai Nagar- 608002, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study was aimed to investigate the cytotoxic effect of ethanol extract of Abrus precatorius. leaves on Raw 264.7 and SK-N-SH cell lines.
Methodology: Soxhlet extraction was carried out using absolute alcohol and subsequently, the profiling of phytoconstituents of ethanol extract was performed by LC-MS analysis.
Results: The results showed that the presence of anthocyanin, phenolic acid, carboxylic acid, amino acid and monoester in ethanol extract of Abrus precatorius. The phytoconstituents such as picolinic acid, N-Acetyl-DL-tryptophan, 3-Hydroxybenzoic acid, kuromanin, aflatoxin G2, monobutyl Phthalate, laurolactam, 4-Dodecylbenzenesulfonic acid, 4-Methoxycinnamic acid, caffeic acid and octyl decyl phthalate were found in ethanol extract. In addition to this, the cytotoxic effect of the ethanol extract was tested on Raw 264.7 and SK-N-SH cell lines using MTT assay. The cytotoxic study revealed that the ethanol extract of Abrus precatorius was non-toxic to Raw 264.7 cell, but it showed a toxic effect on human neuroblastoma cell line, SK-N-SH.
Conclusion: In this study, it was observed that the ethanol extract of Abrus precatorius was non-toxic to Raw 264.7 cell line, but it exhibited strong inhibition on the viability of SK-N-SH cell line.
Keywords: Abrus precatorius, anthocyanin, phenolic acid, cytotoxic, Raw 264.7 and SK-N-SH cell lines