Barringtonia asiatica Seed Extract Induces G1 Cell Cycle Arrest in Saccharomyces cerevisiae and Exhibits Cytotoxicity in A2780 Human Ovarian Cancer Cells

Nilita Sirikit P. Villanueva

Department of Pharmacy, College of Health and Allied Medical Professions, University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines and Faculty of the Graduate School, Centro Escolar University, 9 Mendiola St., San Miguel, Manila, Metro Manila, Philippines and Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Carmela Vannette B. Vicera

Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Sheeny Fane L. Leonida

Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Melissa June Paderog

Department of Pharmacy, College of Health and Allied Medical Professions, University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines and Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Edna M. Sabido

Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Jonel P. Saludes

Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines and Department of Chemistry, College of Liberal Arts, Sciences and Education, University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

Doralyn S. Dalisay *

Faculty of the Graduate School, Centro Escolar University, 9 Mendiola St., San Miguel, Manila, Metro Manila, Philippines and Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines and Department of Biology, College of Liberal Arts, Sciences and Education, University of San Agustin, Gen. Luna Street, Iloilo City, 5000, Philippines.

*Author to whom correspondence should be addressed.


Abstract

Barringtonia asiatica, is traditionally used as a medicinal plant in the Philippines; unfortunately, there is limited evidence supporting its anticancer capability. Thus, our study investigated the cytotoxic effect of B. asiatica seed extract against ovarian carcinoma A2780 (ECACC 93112519) and Saccharomyces cerevisiae as a cancer model organism. Seeds of B. asiatica were freeze-dried and extracted with methanol. The seed extract was investigated for its effect on the cell cycle in S. cerevisiae by utilizing yeast budding experiment and imaging flow cytometry. Its cytotoxicity activity was also tested as well as a live/dead assay was conducted against ovarian cancer cells. Findings revealed the anticancer activity of B. asiatica and its capability to induce G1 cell cycle arrest in S. cerevisiae. Thus, B. asiatica seeds may serve as a potential source of natural compounds towards anticancer drug discovery.

Keywords: Barringtonia asiatica, medicinal plants, cancer yeast model, yeast budding experiment, antiproliferative assay, cytotoxicity


How to Cite

Villanueva, N. S. P., Vicera, C. V. B., Leonida, S. F. L., Paderog, M. J., Sabido, E. M., Saludes, J. P. and Dalisay, D. S. (2021) “Barringtonia asiatica Seed Extract Induces G1 Cell Cycle Arrest in Saccharomyces cerevisiae and Exhibits Cytotoxicity in A2780 Human Ovarian Cancer Cells”, Journal of Pharmaceutical Research International, 33(53A), pp. 372–387. doi: 10.9734/jpri/2021/v33i53A33672.

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