In vivo Evaluation of Nanostructured Lipid Carrier System in Rats Bearing Breast Tumor
Priti Tagde
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida 201301, India.
Kalpana Nagpal *
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida 201301, India.
Giriraj T. Kulkarni
Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad 50090, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
The aim of this work is to study the anti-proliferative potential of two anticancer drugs loaded in nanostructured lipid carriers (NLCs).The maximal inhibition of cell growth by Raloxifene (RLX) & Curcumin (CUM) nanostructured lipid carriers (RLX-CUM-NLCs) was determined by assessing the viability of MDA-MB 231 cells. As far as we know, this is the first research to look at the effects of RLX-CUM-NLCs on DMBA-induced breast carcinogenesis in a rat model. RLX-CUM-NLCs reduced the number of tumors in an in-vivo investigation. After 14 weeks of induction, we discovered a tumor with a 100% incidence rate. The incidence of experimental breast cancer was decreased to 83.33% in the RLX-treated group. In contrast, RLX-CUM-NLCs demonstrated a significant anticancer effect with a 50% incidence in the RLX-CUM-NLCs group. Compared to controls, the RLX-CUM-NLCs therapy did not cause any toxicity in the animals in terms of food intake, body weight, or activity levels until 300 mg/kg BW. The current research shows that the RLX-CUM-NLCs has a chemopreventive impact on DMBA-induced breast cancer in rats by decreasing tumor burden and restoring marker enzymes activity.
Keywords: Nanostructured lipid carriers, antineoplastic activity, tumorigenesis, DMBA