Isolation, Characterization and Antimicrobial Activity of 3β, 22E-Stigmasta-5, 22-dien-3-ol from the Aerial Part of Aeschynomene uniflora E. Mey
J. I. Achika *
Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria
G. I. Ndukwe
Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.
R. G. Ayo
Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Objectives: To isolate, characterize and determine the antimicrobial activity of bioactive compounds present in the petroleum ether fraction of the aerial parts of Aeschynomene uniflora E. Mey.
Methods: Aeschynomene uniflora was collected, identified, dried and pulverized. The pulverized plant material was subjected to Soxhlet extraction using petroleum ether. The petroleum ether extract was subjected to chromatographic techniques which yielded J1. J1 was then subjected to antimicrobial screening using some selected microorganisms. J1 was characterized using IR and NMR spectroscopic techniques.
Results: The structure of the isolated compound was established to be 3β, 22E-Stigmasta-5, 22-dien-3-ol (stigmasterol) using spectroscopic analysis (IR, 1D-NMR). The isolated compound showed significant antimicrobial activity on some selected microorganisms.
Conclusion: The results could justify the use of the aerial parts of Aeschynomene uniflora in traditional herbal medicine for the treatment of various microbial diseases.
Keywords: Aeschynomene uniflora, aerial part, stigmasterol and antimicrobial activity.