Effect of Aegle Marmelos Hydroethanolic Leaf Extract on Expression of Antiapoptotic Markers in Human Melanoma Cells
S. Bhavesh
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, India.
G. Sridevi *
Department of Physiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai-77, Tamil Nadu, India.
J. Selvaraj
Department of Biochemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences Velappanchavadi, Chennai-600 077, India.
S. Preetha
Department of Physiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai-77, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Aegle marmelos commonly known as Bael is a herbal plant. Itis from a family called Rutaceae. It has many medicinal uses: anti-diarrheal, anti- microbial, anti-viral, anti-cancer, chemo-preventive, Ulcer healing and many others.
Aim: To study the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
Objective: The present study investigated the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
Materials and Methods: DMSO and MTT chemicals were purchased from Sigma chemical Pvt Ltd. Trypsin EDTA, FBS, RPMI 1640 medium and PBS, Real time PCR kit was purchased from Canada. Human melanoma cell line (A375) was purchased from NCCS, Pune, India.
Results: The data was analysed statistically by ANOVA and Duncan’s multiple range test with a computer based software (Graph Pad Prism version 5). The percentage of cell viability decreases with the increase in dosage of Aegle marmelos leaf extract.
Conclusion: The study concluded that Aegle marmelos hydro ethanolic leaf extract a novel and innovative herbal drug has a significant effect on the expression of antiapoptotic markers in human melanoma cell lines.
Keywords: Aegle marmelos, innovative, novel, human melanoma, RT-PCR, gene expression