Application of Box–Behnken Design and Desirability Function in the Development and Optimization of Stealth Liposomes of Microtubule Inhibitor
Bandaru Lakshmi Narayana Rao
Department of Pharmacy, Annamalai University Annamalai Nagar, Chidambaram, Tamil Nadu, India.
S. Parimala Krishnan *
Department of Pharmacy, Annamalai University Annamalai Nagar, Chidambaram, Tamil Nadu, India.
Challa Balashekar Reddy
Department of Pharmacy, Annamalai University Annamalai Nagar, Chidambaram, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of the present study was to develop and optimize a Stealth Liposomal Drug Delivery System of microtubule inhibitor using Box–Behnken Design and Desirability function.
Study Design: Development and Optimization of Stealth Liposomes.
Place and Duration of Study: The study was carried out in the Department of Pharmacy, Annamalai University, between September 2020 and May 2021.
Methodology: Stealth Liposomes were prepared by the thin-film hydration method (TFH). The formulation was optimized using Box – Behnken design to study the effect of independent variables, Amount of Egg Phosphatidylcholine (X1), Amount of Cholesterol (X2), and Amount of DSPE-PEG 2000(X3) on dependent variables Entrapment Efficiency (Y1) and In-vitro drug release (Y2).
Results: Entrapment efficiency of the Stealth Liposomes ranges from 56.35 to 84.25%and in-vitro release ranges from 62.38 to 94.26%. The optimized formulation was found using the desirability function to get maximum entrapment with maximum drug release. The optimized formulation showed entrapment efficiency of 80.46% and in-vitro release of 90.11%.
Conclusion: Stealth Liposomal Drug Delivery System for microtubule inhibitor was successfully developed and optimized using desirability function in Design Expert software by a three-factor, three level Box – Behnken design.
Keywords: Stealth liposomal drug delivery, thin film hydration, box – behnken design, desirability function