Evaluation of COX-2 Inhibitor Injurious Effects on Proximal Convoluted Tubular Diameter of Kidney with Favorable Impact of Lycopene in Albino Rats on the Basis of Microscopic Features
Sadia Sundus *
Department of Anatomy, Iqra Medical & Dental, Karachi, Pakistan.
Aisha Hassan
Department of Anatomy, FRPMC, Pakistan.
Ashok Kumar
Department of Anatomy, Shaheed Mohtrmma Benazir Bhutto Medical College, Pakistan.
Shah Jabeen
Department of Physiology, FRPMC, FRPMC, Pakistan.
Nadira Hameed
Department of Anatomy, FRPMC, FRPMC, Pakistan.
Madiha Ajaz
Department of Human Nutrition & Dietetics, Iqra University, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Objective: To analyze the harmful effect of COX-2 inhibitor on proximal convoluted tubular diameter of kidney, amendment by lycopene.
Research Design: Experimental Research.
Abode of Research: Animal House, JPMC, Karachi.
Materials and Methods: 90-120 days old, forty healthy adult male Albino rats of 200-220gm weight were taken for this study and distributed into 4 cliques, set 1was chosen as control, in Set 2 Celecoxib was given 0.05g/1000g by gavage, in set 3 Celecoxib was given0.05g/1000g by gavage with lycopene 0.05g/1000g by gavage and set4 lycopene was given0.05g/1000g by gavage for 30 days. At accomplishment of study, animals were sacrifice and tissues were preserved for staining.
Results: Inset 2 proximal convoluted tubular diameter became distended due to apoptosis of lining epithelial cells. They turn into low cuboidal from simple cuboidal cells along with ill-defined brush border at the luminal surface due to scarce microvilli and had disrupted basement membrane, however renal structure were amended in set3 which were given celecoxib with lycopene.
Conclusion: This study reveals that lycopene amended the apoptotic changes of set2.
Keywords: Celebrex, nephrotoxicity, tubular necrosis, chemo preventive