Antileishmanial Activity of Cassia fistula L., Morus nigra L. and Ziziphus jujuba Mill., Plant Extracts
Kashif Iqbal
*
Department of Pharmacy, The University of Lahore - Islamabad Campus, Islamabad, Pakistan.
Eshwa Dar
Faculty of Pharmacy, University of Lahore, Lahore, Pakistan.
Abdul Rashid Shaikh
Faculty of Pharmacy, University of Lahore, Lahore, Pakistan.
Samar Akhtar
Yusra Institute of Pharmaceutical Sciences, Islamabad, Pakistan.
. Marvi
Department of Pharmacology, University of Balochistan, Quetta, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Aim: To investigate the different antileishmanial activities of extracts of Cassia Fistula L., Morus Nigra L. and Ziziphus Jujuba Mill.
Methodology: In this method, three (03) plants having concentrations between 500 – 2000 µg/mL were subjected to KWH23 strains of L. tropica in which Standard drug was Amphotericin B and have negative control for 24 – 48 hours. To check the in-vivo studies, plant extract was tested on BALB/c mice (Iqbal et al., 2016).
Results: It showed that inhibition (mean) of KWH23 strains at 500, 1000, 1500, 2000 µg/mL after 48 hours were 92.1 ± 0.02, 95.00 ± 0.05, 97.09 ± 0.07 and 98.05 ± 0.05 % respectively. It decreased the lesion size (mean) from 0.8 ± 0.1 mm to 0.40 ± 0.2 mm having significance value p < 0.01 after 8th week, and cure at 200 mg/Kg against intracellular amastigotes in BALB/c mice was 90.00% (95% Cl = 80.05 – 97.00).
Conclusion: The result shows that Ziziphus jujuba Mill. leaves possess significant antileishmanial activity.
Keywords: Ziziphus jujuba Mill., Cassia fistula L., Morus nigra L., Leishmania tropica, promastigotes, amastigotes