The Use of Different Proteins as a Carrier Protein to Obtaining Morphine-Protein Conjugates for ELISA Diagnosis of Drug Addicts
Farkhod Eshboev *
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan and Faculty of Biology National University of Uzbekistan Named after MirzoUlugbek, Tashkent, Uzbekistan.
Elvira Yusupova
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Galina Piyakina
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Sabirdjan Sasmakov
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Jaloliddin Abdurakhmanov
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Shuhrat Khasanоv
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Oybek Ashirov
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
Sherali Kuziev
Faculty of Biology National University of Uzbekistan Named after MirzoUlugbek, Tashkent, Uzbekistan.
Durdona Toshpulatova
Faculty of Biology National University of Uzbekistan Named after MirzoUlugbek, Tashkent, Uzbekistan.
Shakhnoz Azimova
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, M. Ulugbek St. 77, 100170, Tashkent, Uzbekistan.
*Author to whom correspondence should be addressed.
Abstract
Drug addiction is one of the biggest problems of medicine because diagnosis and treatment of drug addiction are difficult compared with some other socially significant diseases. In this study, synthesis and evaluation of four carrier protein-morphine conjugates were experimented. These conjugates were evaluated based on ELISA; soybean protein-based conjugate was selected for further analysis. The total soybean protein was isolated from the local soybean variety and; it was fractioned by the gel-filtration method and their amino acids compositions were studied. After that, the ELISA drug addicts were conducted based on soybean protein-morphine conjugates synthesized with soybean protein fractions. The high molecular weight soybean protein- morphine conjugate showed the highest quality.
Keywords: Drug addiction, morphine-protein conjugates, soybean protein, ELISA, amino acids