Pharmacokinetic and Phytochemical Appraisal of Trigonella foenum and Andrographis lineata in Dimethylnitrosamine Induced Injury in Albino Rats
Sara Zahid *
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Asma Ahmed
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Hasan Akbar Khan
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Fatima Zahid
Department of Pharmacy, The University of Lahore, Islamabad, Pakistan.
Zeeshan Akbar
Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan.
Hafsa Waheed
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Ambreen Abbas
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Trigonelline is a plant alkaloid and Andrographolide is a diterpene lactone, both exhibiting anti-inflammatory, antioxidant and neuroprotective activities. The present study was designed to evaluate the antioxidative and anti-inflammatory activity of the above said compounds in Dimethylnitrosamine induced toxicity in albino rats. Extraction of Trigonella foenum and Andrographis lineata was carried out by using methanol, petroleum ether, ethyl acetate and ethanol assisted with suitable temperature, followed by DPPH scavenging activity (IC-50) of these extracts.Cmax, Tmax, t1/2, CL, Vd and AUC were evaluated as pharmacokinetic parameters by using calibration curves of Andrographolide and Trigonelline. Extracts of T. foenum-graecum and A. lineate have antioxidant activity by inhibiting DPPH (IC50 value was 69.04±3.65% and 71.76±6.99%, respectively) comparable with ascorbic acid (53.99±4.88%). Phytochemical analysis of T. foenum-graecum and A. lineate was found with maximum number of phenols in them, with least recovery in the ethyl acetate extract while maximum phenols were found in T. foenum graecum. All the pharmacokinetic parameters of trigonelline and andrographolide administered in low dose (200 mg/kg and 50 mg/kg respectively) confirmed the better antioxidative activity than that of ascorbic acid used as a potent antioxidant.
Keywords: Antioxidant, bioavailability, secondary metabolites, therapeutic activity, scavenging potential, ascorbic acid