Chemometric Access for RP-HPLC Simultaneous Estimation of Tadalafil and Dapoxetine Applying Response Surface Methodology
S. Jayaseelan *
Department of Pharmaceutical Analysis, the Erode College of Pharmacy, Veepampalayam, Erode, India.
N. Kannappan
Department of Pharmacy, Annamalai University, Chidambram. India.
V. Ganesan
Department of Pharmaceutics, the Erode College of Pharmacy, Veepampalayam, Erode.Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: A RP-HPLC method was developed and validated for simultaneous estimation of Tadalafil and Dapoxetine applying statistical experimental design.
Methodology: Multivariate optimization of the experimental conditions of RP-HPLC method was using Design of experiments. Independent three factors like phosphate buffer pH, mobile phase composition and flow rate were applied to design mathematical models. To study the response surface methodology by using Central composite design (CCD). In depth the effects of these independent factors was studied using CCD. Simultaneously optimize the retention time and resolution of the analytes was applying Desirability function.
Results: The predicted and optimized data from contour picture containing phosphate buffer (pH 3.4) and acetonitrile in the ratio of 40:60%v/v respectively. Flow rate was found to be 0.8 ml/min. Baseline separation of both analytes with run time of less than 10.0 min and good resolution were achieved using these optimum conditions.
Conclusion: Method was validated according to ICH guidelines by using optimized assay conditions. Therefore, the reports distinctly indicated that Quality by design access could be satisfactorily used to optimize RP-HPLC method for simultaneous estimation of Tadalafil and Dapoxetine.
Keywords: RP-HPLC, optimum conditions, central composite design, response surface methodology, tadalafil and dapoxetine