Antiangiogenesis and Anticancer Activity of Leaf and Leaf Callus Extracts from Baccharoides anthelmintica (L.) Moench (Asteraceae)
V. Chinnadurai
Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
K. Kalimuthu *
Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
R. Prabakaran
PSG College of Arts and Science, Coimbatore- 641014, Tamil Nadu, India.
Y. Sharmila Juliet
Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Baccharoides anthelmintica (L.) Moench. is an annual herb distributed throughout India, this plant has high trade value because of its many medicinal properties such as inflammatory swelling, stomachache, diuretic properties, cough, fever, diuretic, leprosy, piles, dropsy, enzyme, ringworm herpes, elephantiasis, incontinence of urine, stomach ache and rheumatism, antimicrobial, antioxidant and anti-cancer. Antiangiogenic activity of ethanol leaf and leaf callus extracts was tested through in vivo CAM (Chorioallantoic membrane) model. Both leaf and leaf derived callus showed higher angiogenic activity 79.33 and 66.0 percentage respectively at 40 µg/mL concentration. The average number of vessels in leaf and leaf derived callus extracts treated CAM was 2 and 3 respectively. Inhibition percentage and vessel number of both the extracts was almost equal in both the concentrations. The cytotoxicity of leaf and leaf derived callus was tested using the MTT assay (HeLa cell line). At a concentration of 300 mg/mL, both the tested samples produced cytotoxic effect as evidenced by the number of dead cells (24.3%, 16.34% respectively).
Keywords: CAM, MTT assay, antiangiogenesis, anticancer, HeLa cell line