A Stability Indicating Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Hydroquinone, Hydrocortisone and Tretenoin in Cream Formulation
Raksha Shilu *
School of Pharmacy, RK University, Rajkot, India and Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.
Pankaj Kapupara
School of Pharmacy, RK University, Rajkot, India.
Meghna Patel
Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: A simple, sensitive, rapid, precise and accurate stability indicating RP-HPLC method has been developed for simultaneous estimation of Hydroquinone, Hydrocortisone and Tretenoin from their Cream Formulation.
Method: The Chromatographic separation was achieved on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 µm) column using a mobile phase consisting of Buffer (pH 4.0) 0.05M potassium dihydrogen ortho phosphate-Methanol in the ratio of 80:20% V/V at a flow rate of 1ml/min and UV detection at λmax 265 nm. The method showed linearity with correlation coefficient of Hydroquinone, Hydrocortisone and Tretenoin was 0.998, 0.998 and 0.996 over the range of 40-120 µg/ml, 20-60 µg/ml and 0.25-0.75 µg/ml respectively.
Result: The retention time was 3 min, 5 min and 6 min for Hydroquinone, Hydrocortisone and Tretenoin respectively. The mean recoveries were found to be in the range of 97.00 –101.00 % for all the components. The method was validated as per the ICH guidelines. The method was validated as per the ICH guidelines.
Conclusion: The method was stable and specific and when sample was stressed under different conditions like Acid, Base, Oxidative, Thermal and Photolytic, no interference of degradants were observed.
Keywords: Hydroquinone, Hydrocortisone, Tretenoin, Stability indicating RP-HPLC, Validation