Determination of Clinical Utility of Novel Biochemical Markers in Osteoarthritis
Ankita Kondhalkar
Department of Biochemistry, Datta Meghe Medical College, Nagpur, India.
Ranjit Ambad
Department of Biochemistry, Datta Meghe Medical College, Shalinitai Meghe Hospital and Research Centre Nagpur-441110 (Datta Meghe Institute of Medical Sciences), India.
Neha Bhatt
Department of Pathology, Datta Meghe Medical College, Shalinitai Meghe Hospital and Research Centre, Nagpur-441110, (Datta Meghe Institute of Medical Sciences), India.
Roshan Kumar Jha
Department of Biochemistry, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha-442001, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Osteoarthritis is a progressive joint disease characterized by loss of articular cartilage, joint bone hypertrophy, subchondral sclerosis, and chemical and morphological alterations in the synovial membrane and joint capsule. Stiffness, soreness, and focused dislocation of the articular cartilage are changes in the disease seen at the last level of OA, as well as synovial inflammation. Pain is a common clinical symptom, especially after prolonged exercise and weight bearing, and stiffness occurs after inactivity. Biologic markers will also play an important role in the production and monitoring of new structure-modifying therapies for osteoarthritis due to their rapid changes in response to treatment.
Aim: We conducted an observational study to estimate biochemical markers in the knee osteoarthritis patients who came to SMHRC Nagpur for a routine visit.
Material and Methods: The study included 60 people who visited Shalinitai Meghe hospital in Nagpur for a health check-up. We were able to keep the two groups apart here. The control group is comprises of Healthy Volunteer, while the study group is made up Knee osteoarthritis patients. Each community consists of 30 patients. COMP, Endoglin, Osteopontin, Hs-CRP: all of these parameters were estimated by commercially available ELISA kit.
Results: The levels of COMP, Endoglin, Osteopontin, and Hs-CRP in the study group were significantly higher than in the control group. In synovial fluid detection, endoglin levels in the sample group are not significantly higher than in the control group. Endoglin levels in the blood increase, as do other parameters.
Conclusion: These findings show a significant increase in the systematic and local development of these biomarkers in the main OA of the knee, as well as the link between disease severity and its production, meaning that they may be involved in OA pathogenesis. Longitudinal studies with repetitive measurements of these biomarkers in plasma and synovial fluid and their interactions with knee pain OA are necessary to track or predict the clinical course of OA and, ultimately, determine their potential role in determining the best time to participate.
Keywords: Osteoarthritis, COMP, synovial fluid, subchondral sclerosis and endoglin