Biotechnology of Microorganisms Growing– Fundamentals for the Development of a Litter Biodestructor
Anna Nikolaevna Gneush
Kuban State Agrarian University (Named After I. T. Trubilin), Kalinina Street 13, Krasnodar, 350044, Russia.
Albina Vladimirovna Luneva
Kuban State Agrarian University (Named After I. T. Trubilin), Kalinina Street 13, Krasnodar, 350044, Russia.
Nadezhda Leonidovna Machneva
Kuban State Agrarian University (Named After I. T. Trubilin), Kalinina Street 13, Krasnodar, 350044, Russia.
Yury Andreevich Lysenko
Kuban State Agrarian University (Named After I. T. Trubilin), Kalinina Street 13, Krasnodar, 350044, Russia.
Maria Vladimirova Aniskina
Kuban State Agrarian University (Named After I. T. Trubilin), Kalinina Street 13, Krasnodar, 350044, Russia.
Marina Nikolaevna Verevkina
Stavropol State Agrarian University, Zootechnicheskiy Lane 12, Stavropol, 355012, Russia.
Magomed Hizrievich Kilyashanov
Essentuki Institute of Management Business and Law, Ermolova Street 2, Yessentuki, 357600, Russia.
Sergey Nikolaevich Povetkin
North Caucasus Federal University, Pushkina Srteet 1, Stavropol, 355009, Russia.
*Author to whom correspondence should be addressed.
Abstract
The purpose of the research work was to select the optimal conditions for the cultivation of microorganisms. As a result of the conducted research work, the modes of growing a nitrogen-fixing culture and a microorganism with high enzymatic activity were selected and worked out. At the same time, the optimal conditions for the cultivation of Azotobacter sp were determined – the temperature optimum for cell accumulation was 30°C, for increased polysaccharide production 20 °C, aeration within 5-10 l/l/min, agitator speed-150 rpm, pH value within 6.0±0.2 units, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml. A cost-effective nutrient medium was selected for growing Pseudomonas sp. molasses-autolysate medium and optimal conditions for growing the culture: cultivation temperature 30-32 °C, aeration 1.0-1.5 l/l/ min, agitator speed 150-200 rpm, pH 6.8-7.2 units, sub-titration 5.0 % KOH, defoaming with adecanol, cultivation time-72 hours, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml.
Keywords: Litter, destructor, microorganisms, cultivation, culture medium, titer, reducing sugars, dynamics, cultivation mode