Exraction of Secondary Metabolites Using Bacillus endopyticus and Its Applications
S. Kalima
*
Department of Microbiology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, India.
K. Thanga Mariappan
Department of Microbiology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, India.
Jesteena Johney
Department of Microbiology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, India.
R. Ragunathan
Department of Microbiology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, India.
*Author to whom correspondence should be addressed.
Abstract
Bacillus Species was isolated from a soil sample, the isolate was cultivated, identified and its culture medium was extracted. Specific media called Gibbon’s media used for the cultivation and confirmation of the bacteria. 16S rRNA study was done for the morphological identification of the isolate. The crude which was collected from Bacillus endopyticus was purified by column chromatography. The antibacterial activity of the crude extract was identified by agar diffusion test by measuring the minimum inhibitory concentration by dilution assay. The purified extract was active against Staphylococcus aureus, Bacillus cerues, Escherichia coli. The study suggest that Bacillus Sps have the potential to produce antibiotics and can be used to control the growth in future. The further work was able to bring the microbial strains under control. The percentage of cell death was calculated the cell death was 97.95%.
Keywords: Bacillus endopyticus, 16S rRNA, gibbon’s media, antimicrobial activity