Anti-Cancerous Efficacy of Alcoholic and Aqueous Extracts from an Endemic Plant Thermopsis turcica on Liver Carcinoma
Muhammad Muddassir Ali *
Department of Biology, Faculty of Science and Literatures, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey and Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
İbrahim Hakkı Ciğerci
Department of Biology, Faculty of Science and Literatures, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Aims: Thermopsis turcica (T. turcica) is endemic to Turkey. Its general medicinal properties are obvious, but no investigations have been made in term of its anticancerous efficacy against liver carcinoma. So, the present study was designed to evaluate the anticancerousanalysis of alcoholic and aqueous from T. turcica in HepG2 cell line.
Methodology: Plant was collected and different extracts (methyl alcohol, ethyl alcohol, and water) of T. turcica were taken. HepG2 cell line was grown and different doses of each extract of this plant was employed on the HepG2. Cytotoxicty test was performed by the MTT assay.
Results: In the MTT cytotoxicity assay, methanol showed the least cell viability at the highest doses compared to other extracts. Aqueous had the least cytotoxic effects.The concentration dependent response was observed by the each extract.The IC50 values for methyl and ethyl alcohol were found 210±15 µg/ml; 220±10 µg/ml, and for aqueous 290±24 µg/ml, respectively.
Conclusion: Various extracts of T. turcica, expressed the anticancerous activity in the HepG2 cells and methyl alcohol extracts had the highest cytotoxic effects.
Keywords: Cytotoxicity, hepatocarcinoma, endemic plant.