Statistical Optimization of Temperature, Concentration, RPM and pH for the Surface Tension of Biosurfactant by Achromobacter Xylos GSR21
Balaji Somesam Upadhyaya
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Golamari Siva Reddy *
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Mallu Maheshwara Reddy
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Sohom Adhikari
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
S. D. Rajkumar
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Akula Niranjan Babu
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Vanga Manav Goud
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Chelikani Sidhartha
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Varakala Nikhil Reddy
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Divyanshu Dhakate
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
N. Konda Reddy
Department of Mathematics, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur Dist, Andhra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Achromobacter xylos strain GSR21 plays a crucial role in bioremediation of fossil fuel contamination, biopharmaceutical, cosmetics, chemical, petroleum refining, petrochemical, food industries and tertiary oil recovery (Microbial enhanced oil recovery). Response surface quadratic models (RSQM) was applied to reinforce the censorious operating conditions for the assembly of Achromobacter xylos strain GSR21. The Response surface method (RSM) was application to determine the best degrees of cycle factors (Temperature, Concentration, RPM, pH). Central composite design (CCD) of RSM was used to contemplate the four factors at five levels, and strain GSR21 Achromobacter xylos fixation was approximate as a reaction. Relapse coefficients predicted by examination and therefore the model was settled. R2 value regard for bio-surfactant (mN/m) attempted to be 0.81, showing that the model fitted well with the explorative results. The mathematical model predicted by simulation of the foreseen updated values, and bio-surfactant surface tension was found 50 mN/m. The foreseen model was matched at 98.8% with the test outcomes coordinated under the perfect conditions. Based on the finding research, temperature-40°C, concentration-1.8 g/l, RPM-180 rev/mint and pH-4 was perceived as compelling fragments for Achromobacter xylos GSR21.
Keywords: Achromobacter xylos GSR21, response surface methodology, central composite design.