Bio Active Compound Analysis of Croton scabiosus Bedd: By HPTLC, GC-MS and Evaluation of Anthelmintic Activity and Anticancer Potential on Lung (A549) and Breast (MCF-7) Cancer Cell Lines
E. Bhargav
RERDS-CPR, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu-515721, Andhra Pradesh, India.
S. Salamma
Department of Botany, Sri Krishnadevaraya University, Ananthapuramu-515003, India.
B. Chandana
Department of Pharmaceutical Chemistry, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu-515721, Andhra Pradesh, India.
S. Harika
Department of Pharmaceutical Chemistry, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu-515721, Andhra Pradesh, India.
M. Vijaya Jyothi
*
Department of Pharmaceutical Chemistry, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu-515721, Andhra Pradesh, India.
B. Ravi Prasad Rao
Department of Botany, Sri Krishnadevaraya University, Ananthapuramu-515003, India.
*Author to whom correspondence should be addressed.
Abstract
Croton scabiosus Bedd. (Euphorbiaceae) is an endemic plant of Andhra Pradesh, India. Local communities use the verdant parts of this plant as an antidote to treat snake bites and as a remedy to heal leucorrhoea in the form of decoction. In the present study the author found to have a perceptible range of alkaloids, coumarins, glycosides, flavonoids, steroids and gallic tannins as secondary metabolites by phytochemical and spectral studies. Since flavonoids and alkaloids are known for remarkable anticancer and anthelmintic activities respectively, the researcher is interested to document the research findings on these activities.The present research is aimed to evaluate bioactive principles present in acetone, chloroform and ethyl acetate extracts of Croton scabiosus Bedd. by chemical tests and spectral analysis. It is also aimed to study the anthelmintic and anticancer potential of these extracts.Acetone, chloroform and ethyl acetate extracts of Croton scabiosus Bedd. were prepared by triple maceration technique. GC-MS and HPTLC analysis were performed to identify chemical constituents. Anthelmintic and anticancer potential was assessed for the three extracts. Owing to the positive results of chemical constituents, the researcher made an attempt to evaluate anthelmintic, cytotoxic potential and anticancer activity on breast adenocarcinoma cells (MCF-7) and lung adenocarcinoma cell line (A549). It was pointed to appraise Croton scabiosus Bedd. extracts have considerable Anthelmintic, cytotoxic and anticancer potential.
Keywords: Anthelmintic, breast adenocarcinoma cells (MCF-7), Croton scabiosus Bedd, cytotoxic potential, lung adenocarcinoma cell line (A549).