A Novel Evidence of Immunological and Molecular Detection of Brucella Species in Camels
Kavitha Manivannan
Department of Genetics, Tharb Camel Hospital, Qatar.
Malathi Ramasamy *
Department of Biotechnology, Government Arts and Science College, Kurumbalur, Perambalur, 621107, Tamilnadu, India.
Hanaa Ahmed
Animal Health Research Institute, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Objectives: Brucellosis is a zoonosis with severe complications for both humans and animals. In this work, we intended to examine the Brucella infection in dromedary camels in Qatar by using different analysis.
Materials and Methods: A total of 203 samples of dromedary camels were randomly collected from the nearby farms in Qatar. Real-time PCR for the genus specific Brucella cell surface salt extractable bcsp31 kDa protein gene were performed on DNA extracted from camel samples. Rose Bengal and rivenol tests were performed to detect the Brucella species. The milk samples were collected from the camels and utilized for the milk ring test.
Results: The outcomes of RT-PCR analysis illustrate the presence of Brucella spp. in 170 samples (83.74%) out of 203 samples. The findings of immunological assays also proved the presence of Brucella spp. such as Rose Bengal (67.14%), ELISA (71.42%), and precipitation assay (65.71) in both serum and blood samples of the dromedary camels, which were collected from the Qatar.
Conclusions: In conclusion, it was clear that the incidence of the brucellosis in camels is significantly rising in Qatar region and there is a need to control the spread of the disease from camels to camels as well as from camels to humans.
Keywords: Brucellosis, dromedary camels, Brucella abortus, Brucella melitensis, RT-PCR, ELISA.