Comparative Study on Antidiabetic Effect of Ethanolic Extract of Jute Leaf on Neonatal Streptozotocin- Induced Type-2 Diabetic Model Rat
Md. Mahabub Ali
Department of Biochemistry, Bangladesh Jute Research Institute, Dhaka–1207, Bangladesh.
Md. Asrafuzzaman
Food Science and Technology Program, BNU-HKBU United International College, Hong Kong Baptist University, Hong Kong. and Asian Network of Research on Antidiabetic Plants (ANRAP), BUHS, Mirpur 1, Dhaka, Bangladesh.
Md. Mahedi Hassan Tusher
Asian Network of Research on Antidiabetic Plants (ANRAP), BUHS, Mirpur 1, Dhaka, Bangladesh. and Department of Pharmacology, Bangladesh University of Health Sciences (BUHS), Mirpur 1, Dhaka, Bangladesh.
Md. Hafizur Rahman
Asian Network of Research on Antidiabetic Plants (ANRAP), BUHS, Mirpur 1, Dhaka, Bangladesh.
Md. Tanvir Rahman
Bangladesh Jute Research Institute, Nashipur, Dinajpur, Bangladesh.
Balaram Roy
Department of Chemistry, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh.
Begum Rokeya *
Asian Network of Research on Antidiabetic Plants (ANRAP), BUHS, Mirpur 1, Dhaka, Bangladesh. and Department of Pharmacology, Bangladesh University of Health Sciences (BUHS), Mirpur 1, Dhaka, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Aim: Functional food and their bioactive compounds have been considered as a new approach for the prevention and management of type 2 diabetes and its complications. According to this approach current study was carried out as an elucidation of antidiabetic properties of Corchorus capsularis and Corchorus olitorius varieties of jute leaf (ethanolic extract) on nSTZ-induced type-2 diabetic rats.
Methodology: The type-2 diabetic model rat was developed by a single intraperitoneal injection of freshly prepared STZ (90 mg/kg/10 ml) in sterile citrate buffer (0.1 M, pH 4.5) to rat pups (48 hour old). After three months, OGTT was performed to select diabetic (FSG > 6.5mmol/L and after 90 min of glucose load > 14 mmol/L) experimental rats. The rats were randomly divided into four groups [DWC, GT, Ext-1 and Ext-2 represent, diabetic water control, glybenclamide treated (20 mg/5 ml/kg body weight), C. capsularis treated and C. olitorius treated group (1.25 g/10 ml/kg body weight) respectively]. One group was kept with normal rats [normal water control, NWC]. The treatment was given once daily or 28 consecutive days. Fasting serum glucose, liver glycogen and lipid profile were estimated by using standard methods.
Results: The results showed that Ext-1 and Ext-2 treated groups gradually decreased serum glucose level (7.15 ±0.67 to 5.94 ± 1.19 and 7.20 ± 0.93 to 5.28 ±1.03 respectively) and reducing effect by Ext-2 was significant (p=0.001). Both extract showed lower liver glycogen level compared with GT group [5.0±2.5 Vs 17.7±6.5 (Ext-1 vs GT) and 7.5±6.4 Vs 17.7±6.5 (Ext-2 vs GT)] and even Ext-1 manifested significant effect (p=0.05). Additionally, lipid profile estimation revealed no significant improvement by the consumption of both the extracts.
Conclusion: On the basis of current investigations, it may be concluded that both variety of jute’s leaf demonstrated hypoglycemic properties in Type 2 diabetic model rats; further in-depth studies are recommended to explore the exact mechanism(s) of hypoglycemic effect.
Keywords: Jute leaf, hyperglycemic, lipid profile, STZ, glibenclazide, T2DM.